Introduction: Streptomyces, gram-positive and aerobic bacteria, are distinguished genus of Actinomycetes. This economically important genus is well studied owing to its capacity in producing more than 70% of antibiotics. In fact, need for novel, safe and more efficient antibiotics is a key challenge to the pharmaceutical industry today, moreover, increase in opportunistic infections in the immune compromised host has influenced this demand. Nowadays, evaluating morphological and biochemical differences as well as studying streptomyces genetic diversity via molecular indicators seem to be the most common method for screening this genus. Methods: In this research we evaluate the potential of antibiotic production and characterize the UV and FTIR spectroscopy and HPLC (High performance liquid chromatography) analysis pattern of streptomyces from various locations in northwest of Iran. Regarding this, 30 soil samples were collected randomly from different zones of northwest region of Iran. Then, following the extraction of secondary metabolite, the UV and FTIR spectroscopy analysis was carried out for characterization of the various extracts. Results: Considering the coordinate analysis of UV and FTIR spectroscopy pattern, the isolate G614C1 with substantial antimicrobial activity exhibited absorption at 3411 cm-1 which is indicator of hydroxyl groups, absorption at 2856 and 2915 cm-1 indicating hydrocarbon chassis, and absorption at 1649 cm-1 indicating a double bond of polygenic compound. Conclusion: These results highlight the importance of streptomyces isolates in antibiotic production. HPLC confirmed the production when compared with standards.