ISSN: 2228-5660    eISSN: 2228-5652  
Bioimpacts. 2016;6(4):219-223.
doi: 10.15171/bi.2016.29
PMID: 28265538
PMCID: PMC5326670
Scopus id: 85013031052

Short Communication

Lactobacillus species mediated synthesis of silver nanoparticles and their antibacterial activity against opportunistic pathogens in vitro

Inna Garmasheva 1 * , Nadezhda Kovalenko 1, Sergey Voychuk 1, Andriy Ostapchuk 2, Olena Livins’ka 1, Ljubov Oleschenko 1

1 Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine
2 Laboratory of Biological Polymer Compounds, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine

Abstract

Introduction: Application of lactic acid bacteria for synthesis of silver (AG) nanoparticles (NPs) could be a good ecological friendly alternative to chemical and physical methods. The objective of this study was to investigate the biosynthesis of silver NPs using Lactobacillus strains and to compare their monosaccharide composition of capsular exopolysaccharides and the antibacterial activity of synthesized nanoparticles.
Methods: The washed cells of 22 Lactobacillus strains were used for in vitro silver nanoparticle biosynthesis from silver nitrate solution. The NPs formation was confirmed by UV-visible spectroscopy and transmission electron microscopy (TEM) analysis. TEM micrographs were used for the evaluation of NPs size. The monosaccharide composition of capsular exopolysaccharides was determined using GC/MS analysis. The antimicrobial activity was determined by agar well diffusion assay.
Results: The capsular layers of Lactobacillus strains contained heteropolysaccharides that were composed mostly of glucose, mannose, galactose and rhamnose in a different molar ratio. It was found that Ag NPs with large size (30.65 ± 5.81 nm) obtained from L. acidophilus 58p were more active against S. epidermidis, E. coli, K. pneumonia, S. flexneri and S. sonnei compared with Ag NPs from L. plantarum 92T (19.92 ± 3.4 nm).
Conclusion: The size and antibacterial activities of Ag NPs were strain-dependent and such characteristics may be due to the capsular biopolymer composition of Lactobacillus strains used for Ag NPs synthesis.

First name
 
Last name
 
Email address
 
Comments
 
Security code


Submitted: 18 Aug 2016
Revised: 20 Oct 2016
Accepted: 06 Nov 2016
First published online: 29 Dec 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Abstract Page Views: 271
PDF Downloads: 246
Full Text Views: 154