Logo-bi
Bioimpacts. 2014;4(4): 191-198.
doi: 10.15171/bi.2014.019
PMID: 25671175
PMCID: PMC4298710
Scopus ID: 84920984152
  Abstract View: 1523
  PDF Download: 1056

Original Research

Geno/cytotoxicty and Apoptotic Properties of Phenolic Compounds from the Seeds of Dorema Glabrum Fisch. C.A

Morteza Eskandani 1,2, Elmira Dadizadeh 3, Hamed Hamishehkar 4, Hossein Nazemiyeh 1,3*, Jaleh Barar 1,3* ORCID logo

1 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
4 Drugs Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Authors: Email: nazemiyehh@tbzmed.ac.ir; Email: bararj@yahoo.com

Abstract

Introduction: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anticancer potency of bioactive compounds from the seeds of Dorma glabrum. Methods: Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds.
Results: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 µg/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments.
Conclusion: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 1377

Your browser does not support the canvas element.


PDF Download: 1056

Your browser does not support the canvas element.

Submitted: 15 Dec 2014
ePublished: 23 Aug 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)