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Bioimpacts. 2012;2(2): 91-95.
doi: 10.5681/bi.2012.013
PMID: 23678446
PMCID: PMC3648926
Scopus ID: 84865375417
  Abstract View: 2592
  PDF Download: 1106

Original Research

Electrochemical Impedance Spectroscopic Sensing of Methamphetamine by a Specific Aptamer

Mohsen Ebrahimi 1,2,3, Mohammad Johari-Ahar 3,4, Hossein Hamzeiy 1,3*, Jaleh Barar 3,5 ORCID logo, Omid Mashinchian 3, Yadollah Omidi 3,5* ORCID logo

1 Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
5 Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
*Corresponding Authors: Email: hhamzeiy@hotmail.com; Email: yomidi@mail.med.upenn.edu

Abstract

Introduction: Electrochemical impedance spectroscopy (EIS) is a simple and highly sensitive technique that can be used for evaluation of the aptamer-target interaction even in a label-free approach. Methods: To pursue the effectiveness of EIS, in the current study, the folding properties of specific aptamer for methamphetamine (METH) (i.e., aptaMETH) were evaluated in the presence of METH and amphetamine (Amph). Folded and unfolded aptaMETH was mounted on the gold electrode surface and the electron charge transfer was measured by EIS. Results: The Ret of methamphetamine-aptaMETH was significantly increased in comparison with other folding conditions, indicating specific detection of METH by aptaMETH. Conclusion: Based on these findings, methamphetamine-aptaMETH on the gold electrode surface displayed the most interfacial electrode resistance and thus the most folding situation. This clearly indicates that the aptaMETH can profoundly and specifically pinpoint METH; as a result we suggest utilization of this methodology for fast and cost-effective identification of METH.
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Submitted: 03 Apr 2012
Revision: 15 Apr 2012
Accepted: 20 Apr 2012
ePublished: 20 May 2012
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