Tabriz University of Medical Sciences BioImpacts 2228-5652 12 4 2022 07 01 Designing multi-epitope based peptide vaccine candidates against SARS-CoV-2 using immunoinformatics approach 359 370 10.34172/bi.2022.23769 EN Ysrafil Ysrafil https://orcid.org/0000-0002-5980-7525 Zulfiayu Sapiun https://orcid.org/0000-0003-2246-2778 Indwiani Astuti https://orcid.org/0000-0001-9817-2561 Mohammad Anas Anasiru Nangsih Sulastri Slamet https://orcid.org/0000-0002-7515-1175 Hartati Hartati https://orcid.org/0000-0002-4426-6033 Fadli Husain Sukmawati Ahmad Damiti https://orcid.org/0000-0003-1379-6864 Journal Article 10.34172/bi.2022.23769 2021 04 07 2021 08 04 Introduction: The current incidence of the novel coronavirus disease has shown only small reductions of cases and has become a major public health challenge. Development of effective vaccines against the virus is still being encouraged such as multi-epitope vaccines designed from the components of SARS-CoV-2 including its spike, nucleocapsid and ORF1a proteins. Since the addition of adjuvants including HABA protein and L7/L12 ribosomal are considered helpful to increase the effectiveness of the designed vaccine, we proposed to design multiepitope vaccines by two different adjuvants. Methods: We used the IEDB server to predict BCL and TCL epitopes that were characterized using online tools including VaxiJen, AllPred and IL-10 Prediction. The selected epitopes were further constructed into multiepitope vaccines. We also added two different adjuvants to the vaccine components in order to increase the effectiveness of the vaccines. The 3D-structured vaccines were built using trRosetta. They were further docked with different Toll-like-receptors (TLR 3, 4 and 8) and the entry receptor of SARS-CoV-2, ACE2 using ClusPro, PatchDock and refined by FireDock. All structures were visualized by USCF Chimera and PyMOL. Results: In this study, we succeeded in designing two different candidate vaccines by the addition of HABA protein and L7/L12 ribosomal as adjuvants. The two vaccines were almost equally good in terms of their physicochemical properties and characteristics. Likewise, their strong interactions with TLR3 4, 8 and ACE2 show the lowest energy level of both was estimated at more than -1,000. Interactions of vaccines with ACE2 and TLRs are essential for activation of immune responses and production of antibodies. Conclusion: The two designed and constructed multiepitope vaccine have good characteristics and may have the potential to activate humoral and cellular immune responses against SARS-CoV-2. Further research is worth considering to confirm the findings of this study.