Tabriz University of Medical Sciences BioImpacts 2228-5652 14 3 2024 05 01 Regulation of cell fate by cell imprinting approach in vitro 29945 29945 10.34172/bi.2023.29945 EN Farkhonde Hasannejad https://orcid.org/0000-0001-9722-9116 Leila Montazeri João F Mano https://orcid.org/0000-0002-2342-3765 Shahin Bonakdar https://orcid.org/0000-0001-8759-3790 Ahmad ‎ Fazilat Journal Article 10.34172/bi.2023.29945 2023 06 10 2023 09 19 Cell culture-based technologies are widely utilized in various domains such as drug evaluation, toxicity assessment, vaccine and biopharmaceutical development, reproductive technology, and regenerative medicine. It has been demonstrated that pre-adsorption of extracellular matrix (ECM) proteins including collagen, laminin and fibronectin provide more degrees of support for cell adhesion. The purpose of cell imprinting is to imitate the natural topography of cell membranes by gels or polymers to create a reliable environment for the regulation of cell function. The results of recent studies show that cell imprinting is a tool to guide the behavior of cultured cells by controlling their adhesive interactions with surfaces. Therefore, in this review we aim to compare different cell cultures with the imprinting method and discuss different cell imprinting applications in regenerative medicine, personalized medicine, disease modeling, and cell therapy.