Tabriz University of Medical Sciences BioImpacts 2228-5652 15 1 2025 01 19 Anticancer impacts of the unicellular cyanobacterium Chroococcus turgidus bioactive compounds in colorectal adenocarcinoma 30867 30867 10.34172/bi.30867 EN Hamieh Goshtasbi https://orcid.org/0000-0002-4306-7243 Azam Safary https://orcid.org/0000-0002-8997-2003 Ali Movafeghi https://orcid.org/0000-0002-4134-6986 Jaleh Barar https://orcid.org/0000-0002-0105-919X Mostafa Akbarzadeh-Khiavi https://orcid.org/0000-0002-1552-6537 Yadollah Omidi https://orcid.org/0000-0003-0067-2475 Journal Article 10.34172/bi.30867 2024 11 26 2025 03 17 Introduction: Microalgae and cyanobacteria are promising sources of bioactive compounds with antioxidant and anticancer properties. The cyanobacterium Chroococcus turgidus has been studied for its potential antioxidant, anti-inflammatory, antibacterial, antiviral, and anticancer effects. This study investigates its anticancer effects on colorectal cancer (CRC) at the cellular and molecular levels. Methods: The metabolites of C. turgidus were screened using the Folin–Ciocalteu reagent and GC-MS. Antioxidant activity was assessed using the DPPH assay. The biological effects of methanolic extract (ME) were evaluated using MTT assay, Annexin V/PI staining, DAPI staining, and western blotting. Cells were treated with ME at concentrations ranging from 5 to 500 µg/mL for 24 and 48 hours, with the IC50 values determined at 373 µg/mL and 291 µg/mL, respectively. Results: ME contained bioactive compounds such as phenols, flavonoids, and anthocyanins. Identified fatty acids included palmitic acid ethyl ester (15.53%), 1-bromo-11-iodoundecane (2.31%), undecanoic acid 2,8-dimethyl methyl ester (6.62%), oleic acid (6.47%), and 7-dehydrocholesterol (7.97%). ME inhibited SW480 cell proliferation in a dose- and time-dependent manner and induced nuclear fragmentation, chromatin remodeling, and apoptosis. Annexin V/PI staining confirmed apoptosis as the dominant mode of cell death. Western blot analysis showed increased Bax and decreased Bcl2 expression, supporting its pro-apoptotic activity. Conclusion: C. turgidus may serve as a potential therapeutic agent for gastrointestinal cancers through its ability to modulate the Bax/Bcl2 pathway and promote apoptosis. These findings highlight its novel anticancer effects and support further preclinical investigations.