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Bioimpacts. 2018;8(1): 5-12.
doi: 10.15171/bi.2018.02
PMID: 29713597
PMCID: PMC5915708
Scopus ID: 85044304206
  Abstract View: 4964
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Original Research

The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats

Navid Hosseini Mansoub 1*, Mehmet Gürdal, Elif Karadadaş, Hilal Kabadayi, Seda Vatansever, Gulinnaz Ercan

1 Department of Medical Biochemistry, Faculty of Medicine, Ege University, Izmir, 35100, Turkey
2 Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Manisa, 45200, Turkey
3 Experimental Health Sciences Research Center, Near East University, Mersin, 33010, Turkey
4 Department of Stem Cell, Institute of Health Sciences, Ege University, Izmir, 35100, Turkey
*Corresponding Author: *Corresponding authors: Navid Hosseini Mansoub, Email: n.h.mansoub@gmail.com; Gülinnaz Ercan, Email: , Email: gulinnaz.ercan@ege.edu.tr

Abstract

Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 and VEGF-α as wound healing markers at gene expression level.

Method: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocine (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On 3rd, 7th, 10th and 14th days after treatment, wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and untreated diabetic rats to analyze gene expression levels of wound healing markers by qPCR.

Results: We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1α2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to combined PRP and KLCs-treated group.

Conclusion: We demonstrated that applying PRP and KLCs in combination has a greater potential for treatment of diabetic burn wounds.

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Submitted: 07 Sep 2017
Revision: 30 Oct 2017
Accepted: 08 Nov 2017
ePublished: 18 Nov 2017
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