Association of CRP gene polymophism with CRP levels and coronary artery disease in type 2 diabetes in Ahwaz, Southwest of Iran

Abstract

 Introduction: We evaluated the association between four polymorphisms in the CRP gene with plasma C-reactive protein (CRP) levels,prevalence and severity of  coronary artery  disease(CAD) in type 2 diabetes mellitus  (T2DM)patients .    Methods: We performed coronary angiography for 308  T2DM patients and classified them into two groups: T2DM with CAD and T2DM without CAD, all patient come from Ahwaz, Iran. Plasma levels of CRP, glucose and lipid profile were measured. Genotyping was performed by PCR/RFLP, and the severity of coronary artery  disease was determined by Gensini score.  Results: The GG genotype of SNP rs279421 was associated with  increased risk CAD (OR = 2.38; 95%CI: 1.12- 5.8; P = 0.02)and CA,TT,TA genotypes and A allele of SNP rs3091244 and GA genotypes and A allele of SNP rs3093062 were significantly associated with increased CRP levels.None genotypes or alleles was not associated with Gensini score. We found that the haplotype 7 (AGCG) was associated with decreased  risk of CAD (OR = 0.11; 95%CI: 0.02, 0.66; P = 0.017) . The Gensini score was correlated  with increased levels of CRP, only in CAD group. Conclusion: Although genetic polymorphisms were influenced on plasma CRP levels but none of the alleles and genotypes raising or falling C-reactive protein levels was consistently associated with an increased prevalence of CAD or protected from that.

Keywords: Type2diabetes mellitus, C-reactive protein, Polymorphism, Coronary artery disease, Gensini score