Adel Rezaei-Moghadam
1, Daryoush Mohajeri
2, Behnam Rafiei
3, Rana Dizaji
4, Asghar Azhdari
5, Mahdi Yeganehzad
5, Maryamossadat Shahidi
6, Mohammad Mazani
7*1 Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Department of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, India
4 Department of Pharmacology and Toxicology, Zanjan University of Medical Sciences, Zanjan, Iran
5 Department of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
6 Department of Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran
7 Department of Biochemistry, Ardabil University of Medical Science, Ardabil, Iran
Abstract
Introduction: Pathogenic
role of free radicals are well known in various metabolic diseases.
They originate from internal and external sources of body. Essential
roles of antioxidant defense system for cellular redox regulation and
free radical scavenging activity were described in this study. Many in
vitro investigations have shown that turmeric (TE) and carrot seed
extract (CSE) exhibits to possess antioxidant activities. In this study,
we evaluated the antioxidant potentials of ethanolic TE and CSE based
on in vivo experiment in the rats. Methods: Animals were assigned to six groups: the 1st and 2nd groups were control groups
and 2nd group received 0.2 ml dimethyl sulphoxide as vehicle treated
group; other four experimental groups received different doses of TE
(100, 200 mg/kg b.w.) and CSE (200, 400 mg/kg b.w.) by gavages,
respectively for a period of one month. The indicators of oxidative
stress, lipids peroxidation, markers of hepatocyte injury and biliary
function markers were measured. Results: The
levels of superoxide dismutase, catalase, and glutathione peroxidase
were significantly stimulated in the hepatic tissue of treatment groups.
The malondialdehyde contents of liver tissue were significantly reduced
in the groups fed with TE and CSE. Serum levels of alanine
aminotransferase, aspartate aminotransferase and alkaline phosphatase,
in treated groups were found to be significantly decreased, whereas
albumin and total protein increased as compared to the control groups
(P<0.05). Conclusion: this
study showed that the regular intake of TE and CSE through the diet can
improve antioxidant status and inhibit peroxidation activity in the
liver tissue so that using these extracts may protect tissue oxidative
stress.