Bioimpacts. 2011;1(1): 47-52.
doi: 10.5681/bi.2011.007
PMID: 23678407
PMCID: PMC3648947
Scopus ID: 84876728566
  Abstract View: 2600
  PDF Download: 1690

Original Research

Characterization of Streptomyces Isolates with UV, FTIR Spectroscopy and HPLC Analyses

Hadi Maleki-Kakelar*

1 Research Centre for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
2 Biology Department, Faculty of Science, University of Tabriz, Tabriz, Iran


Introduction: Streptomyces, gram-positive and aerobic bacteria, are distinguished genus of Actinomycetes. This economically important genus is well studied owing to its capacity in producing more than 70% of antibiotics. In fact, need for novel, safe and more efficient antibiotics is a key challenge to the pharmaceutical industry today, moreover, increase in opportunistic infections in the immune compromised host has influenced this demand. Nowadays, evaluating morphological and biochemical differences as well as studying streptomyces genetic diversity via molecular indicators seem to be the most common method for screening this genus. Methods: In this research we evaluate the potential of antibiotic production and characterize the UV and FTIR spectroscopy and HPLC (High performance liquid chromatography) analysis pattern of streptomyces from various locations in northwest of Iran. Regarding this, 30 soil samples were collected randomly from different zones of northwest region of Iran. Then, following the extraction of secondary metabolite, the UV and FTIR spectroscopy analysis was carried out for characterization of the various extracts. Results: Considering the coordinate analysis of UV and FTIR spectroscopy pattern, the isolate G614C1 with substantial antimicrobial activity exhibited absorption at 3411 cm-1 which is indicator of hydroxyl groups, absorption at 2856 and 2915 cm-1 indicating hydrocarbon chassis, and absorption at 1649 cm-1 indicating a double bond of polygenic compound. Conclusion: These results highlight the importance of streptomyces isolates in antibiotic production. HPLC confirmed the production when compared with standards.
Keywords: UV Spectroscopy, FTIR Spectroscopy, Streptomyces, Antimicrobial Activity
First Name
Last Name
Email Address
Security code

Abstract View: 2600

Your browser does not support the canvas element.

PDF Download: 1690

Your browser does not support the canvas element.

Submitted: 11 Jan 2011
ePublished: 22 Jan 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)