Sonu Gandhi
1*, Pankaj Suman
1, Ashok Kumar
2, Prince Sharma
3, Neena Capalash
3, C. Raman Suri
41 Amity Institute of Biotechnology, Amity University, Noida, India
2 CSIR- Institute of Genomics and Integrative Biology, Delhi, India
3 Department of Microbiology and Biotechnology, Panjab University, Chandigarh, India
4 CSIR- Institute of Microbial Technology, Chandigarh, India
Abstract
Introduction: Immunosensor
for illicit drugs have gained immense interest and have found several
applications for drug abuse monitoring. This technology has offered a
low cost detection of narcotics; thereby, providing a confirmatory
platform to compliment the existing analytical methods.
Methods: In
this minireview, we define the basic concept of transducer for
immunosensor development that utilizes antibodies and low molecular mass
hapten (opiate) molecules.
Results: This
article emphasizes on recent advances in immunoanalytical techniques
for monitoring of opiate drugs. Our results demonstrate that high
quality antibodies can be used for immunosensor development against
target analyte with greater sensitivity, specificity and precision than
other available analytical methods.
Conclusion: In
this review we highlight the fundamentals of different transducer
technologies and its applications for immunosensor development currently
being developed in our laboratory using rapid screening via
immunochromatographic kit, label free optical detection via enzyme,
fluorescence, gold nanoparticles and carbon nanotubes based
immunosensing for sensitive and specific monitoring of opiates.