Bioimpacts. 2020;10(1): 17-25.
doi: 10.15171/bi.2020.03
PMID: 31988853
PMCID: PMC6977593
Scopus ID: 85078964902
  Abstract View: 357
  PDF Download: 264
  Full Text View: 145

Original Research

Kinetic and thermodynamic study of beta-Boswellic acid interaction with Tau protein investigated by surface plasmon resonance and molecular modeling methods

Hossein Haghaei 1 ORCID logo, Seyed Rafie Aref Hosseini 1 ORCID logo, Somaieh Soltani 2, Farzaneh Fathi 3 ORCID logo, Farzad Mokhtari 4 ORCID logo, Saeed Karima 4 ORCID logo, Mohammad-Reza Rashidi 2,3 * ORCID logo

1 Nutrition and Food Sciences Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
2 Pharmacy Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
3 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
4 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran


Introduction: Beta-Boswellic acid (BBA) is a pentacyclic terpene which has been obtained from frankincense and its beneficial effects on neurodegenerative disorders such as Alzheimer’s disease (AD) have been addressed.
Methods: In the present study, thermodynamic and kinetic aspects of BBA interaction with Tau protein as one of the important proteins involved in AD in the absence and presence of glucose has been investigated using surface plasmon resonance (SPR) method. Tau protein was immobilized onto the carboxy methyl dextran chip and its binding interactions with BBA were studied at physiological pH at various temperatures. Glucose interference with these interactions was also investigated.
Results: Results showed that BBA forms a stable complex with Tau (KD=8.45×10-7 M) at 298 K. Molecular modeling analysis showed a hydrophobic interaction between BBA and HVPGGG segment of R2 and R4 repeated domains of Tau.
Conclusion: The binding affinity increased by temperature enhancement, while it decreased significantly in the presence of glucose. Both association and dissociation of the BBA-Tau complex were accompanied with an entropic activation barrier; however, positive enthalpy and entropy changes revealed that hydrophobic bonding is the main force involved in the interaction.
Keywords: Beta Boswellic acid, Tau protein, Kinetic study, Surface plasmon resonance
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Submitted: 21 Jan 2019
Revision: 16 Apr 2019
Accepted: 17 Apr 2019
ePublished: 22 May 2019
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