Tayibeh Ghaffari
1, Mohammad Nouri
1, Ebrahim Irannejad
2, Mohammad-Reza Rashidi
3*1 Nutrition Research Center, Medical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
2 Academic Center for Education, Culture and Research (ACECR), University of Tabriz, Tabriz, Iran
3 Research Center for Pharmaceutical Nanotechnology, Faulty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Introduction:
The aim of the present study was to assess the effects of vitamin E and
selenium supplementation on serum paraoxonase (PON1) activity, lipid
peroxidation and antioxidant defense in streptozotocin-induced diabetic
rats. Methods: Thirty two female Sprague
Dawley rats were divided into 3 groups: the control group (n=8) received
a standard diet; streptozotocin (STZ)-induced diabetic rats (n=12),
received corn oil and physiological solution; and vitamin E and selenium
supplemented diabetic rats (n=12) were treated with oral administration
of vitamin E (300 mg/kg) and sodium selenite (0.5 mg/kg) once a day for
4 weeks. Results: Significantly lower total
antioxidant status (TAS), PON1and erythrocyte SOD activities and a
higher fasting plasma glucose level were observed in the diabetic rats
compared to the control. A significant increase in SOD and GPX
activities in vitamin E and selenium supplemented diabetic group was
observed after 5 weeks of the experiment. Compared to the normal rats,
malondialdehyde (MDA) and oxidized LDL (Ox-LDL) levels were higher in
the diabetic animals; however, these values reduced significantly
following vitamin E and selenium supplementation. Conclusion:
Vitamin E and selenium supplementation in diabetic rats has
hypolipidemic, hypoglycemic and antioxidative effects and may slow down
the progression of diabetic complications through its protective effect
on PON1 activity and lipoproteins oxidation.