Saeed Sadigh-Eteghad
1*, Hossein Tayefi-Nasrabadi
2, Zahra Aghdam
3, Habib Zarredar
4, Dariush Shanehbandi
5, Leila Khayyat
4, Seyyed-Hamed Seyyed-Piran
61 Department of Molecular Neurobiology, Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Biochemistry, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
3 Department of chemistry, Tabriz Branch, Payame Noor University, Tabriz, Iran
4 Department of Microbiology, Faculty of Science, Islamic Azad University of Lahijan, Lahijan, Iran
5 Department of Biotechnology, Faculty of Agriculture, Payame Noor University of Tehran, Karaj, Iran
6 Department Animal Science, Faculty of Agriculture, Islamic Azad University, Branch of Shabestar, Shabestar, Iran
Abstract
Introduction: This research investigates the possible potential of Rosa canina (RC) as an immunomodulator in rats and its effects on some biochemical parameters. Methods:
In this experiment, 45 male Wistar rats were obtained and divided into
three groups (n = 15). These groups received normal saline (10 mg/kg),
RC fruit extract (250 mg/kg) and RC fruit extract (500 mg/kg) as oral
gavages every day for a period of four weeks, respectively. After
obtaining blood samples (at the end of each week), differential white
blood cell (WBC) counts, phagocyte activity (number), alanine
aminotransferase (ALT), aspartate aminotransferase (AST), alkaline
phosphates (ALP) albumin and globulins levels of samples were obtained.
The malondialdehyde (MDA) and glutathione (GSH) levels in the serum were
determined only in day 28 of study. The radical scavenger activity
(RSA) of the RC extract was measured spectrophotometrically. Results:
the gamma globulin level, neutrophil and monocyte counts and phagocyte
activity increased significantly in comparison with the normal saline
group. ALT, AST and ALP had not significantly differences in compared to
control group. RC extract significantly increased thiobarbituric acid
reactive substances (TBARS) and also decreased GSH levels in comparing
to control group in day 28. Conclusion: the data suggest that the RC extract has been used in traditional medicine might have immunomodulatory effects.