Solmaz Asnaashari
1, Elmira Dadizadeh
2, Amir Hossein Talebpour
3, Morteza Eskandani
2, Hossein Nazemiyeh
2*1 Department Drug Applied Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
3 East Azerbaijan Research Center for Agriculture and Natural Resources, Tabriz, Iran
Abstract
Introduction: Dorema glabrum
is an endangered species that grow in Transcaucasia and North West of
Iran. The plant has extensive uses e.g. as an herbal remedy or food
additive in these regions. The chemical composition of hydrodistilled
oil of D. glabrum growing in Iran was analyzed by GC-MS for the first time. Methods: The
essential oil of air-dried roots was obtained by hydrodistillation
using a Clevenger type apparatus. The oil was subjected to GC-MS
analysis and its free radical scavenging properties were determined by
DPPH method. Results: Thirty four constituents were identified that represented 81.6% of the total oil. The main compounds were delta-Cadinene
(12.77%), beta-bisabolene (7.48%), alpha-Fenchyl acetate (6.32%),
Copaene (5.68%) and Cubenol (5.42%). The essential oil had weak free
radical scavenging properties with the RC50 value of 2.24 mg/mL. Conclusion: Present work is the first report on chemical composition of the essential oil obtained from D. glabrum roots. GC-MS Analysis showed that the oil was rich in sesquiterpenes. It deems that weak free radical scavenging activity of the oil is due to absence of potent antioxidant compounds.