Mohammad Pourhassan-Moghaddam
1,2,3, Behrouz Farhadi
2, Kazem Nejati-Koshki
1*1 Department of Medical Biotechnology, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Introduction: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Therefore, it is necessary to use new expression systems to overcome the indicated challenges. Methods: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors. Results: After designing, these vectors could be applied to produce complex human recombinant proteins in the human cancer cell lines as production hosts. Conclusion: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.