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Bioimpacts. 2020;10(1): 37-43.
doi: 10.15171/bi.2020.05
PMID: 31988855
PMCID: PMC6977594
Scopus ID: 85078964326
  Abstract View: 329
  PDF Download: 235
  Full Text View: 125

Original Research

Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells

Roya Hesari 1 ORCID logo, Mina Keshvarinia 2, Mahboubeh Kabiri 3, Iman Rad 4 ORCID logo, Kazem Parivar 2, Hoorieh Hoseinpoor 4, Rezvan Tavakoli 4, Masoud Soleimani 5, Fatemeh Kouhkan 4 ORCID logo, Soheila Zamanluee 6, Hana Hanaee-Ahvaz 4 * ORCID logo

1 Institute of Materials and Biomaterials, Tehran, Iran
2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
4 Stem Cell Technology Research Center, Tehran, Iran
5 Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
6 Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β.
Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays.
Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively.

Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.

Keywords: Calcium deposition, Chondrogenesis, Mesenchymal stem cells, Transforming growth factor-beta, Platelet rich plasma
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Submitted: 05 Aug 2019
Revision: 23 Sep 2019
Accepted: 12 Oct 2019
ePublished: 02 Nov 2019
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