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Bioimpacts. 2018;8(1): 53-58.
doi: 10.15171/bi.2018.07
PMID: 29713602
PMCID: PMC5915709
Scopus ID: 85044261366
  Abstract View: 3260
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Short Communication

Chemiluminescence based immunoassay for the detection of heroin and its metabolites

Smritee Singh 1, Priya Mishra 1,2, Ivneet Banga 1, Avanish S Parmar 3, Prem Prakash Tripathi 4, Sonu Gandhi 1*

1 Amity Institute of Biotechnology, Amity University, Sector-125, Noida-201313, India
2 Amity Institute of Neurophyscology and Neurosciences, Amity University, Sector-125, Noida-201313, India
3 Department of Physics, Indian Institute of Technology-BHU, Varanasi-221005, India
4 CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata, West Bengal-700032, India
*Corresponding Author: Email: sonugandhi@gmail.com

Abstract

Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples.

Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples.

Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL.

Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs.

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Submitted: 03 Aug 2017
Revision: 10 Oct 2017
Accepted: 22 Oct 2017
ePublished: 30 Oct 2017
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