Logo-bi
Bioimpacts. 2018;8(4): 253-261.
doi: 10.15171/bi.2018.28
PMID: 30397580
PMCID: PMC6209832
Scopus ID: 85056115526
  Abstract View: 2800
  PDF Download: 1549
  Full Text View: 1109

Original Research

Characterization and partial purification of an antibacterial agent from halophilic actinomycetes Kocuria sp. strain rsk4

Ravi Ranjan Kumar 1*, Vasantba J Jadeja 2

1 Department of Biotechnology, Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India
2 Department of Microbiology, Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India
*Corresponding Author: Email: raviranjan@vsc.edu.in

Abstract

Introduction: The inevitable rise of antibiotic-resistant bacteria is a global health problem. These pathogens erode the utility of available antibiotics. Staphylococcus aureus is one of the major causes of community-acquired infections. The aim of work was to evaluate the marine actinomycetes for production of the antibacterial agent against pathogens.

Methods: Halophilic actinomycetes were isolated, characterized and screened for production of antibacterial agent against pathogenic bacteria. The antibacterial compounds were extracted by solvent extraction and separated by TLC based bioautography. Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. The active fraction was characterized by spectroscopy techniques. The minimum inhibitory concentration of antibiotic was determined against pathogens.

Results: A new halophilic actinomycetes strain rsk4 was isolated from marine water. It was designated as Kocuria sp. based on the physiological, biochemical and 16S rDNA sequencebased characters. It was able to produce broad-spectrum antibacterial compound and exhibited significant inhibitory activities against antibiotic-resistant S. aureus. The antibacterial compound was secreted optimally at 5% NaCl and neutral pH in the starch casein medium during stationary phase. The crude ethyl acetate extract was separated by chloroform-methanol, 24:1, v/v having Rf value 0.45. Bioassay of HPLC fractions confirms the presence of antibiotics picks at retention time: 3.24 minutes. The UV-Visible and mass spectra of the compound revealed that the active compound was different from other known antibiotics. The lowest minimum inhibitory concentration was recorded against S. aureus (30 µg/mL).

Conclusion: The result suggests that a broad-spectrum antibacterial compound obtained from halophilic actinomycetes is effective against pathogenic bacteria. This compound may be a good alternative treatment against antibiotic-resistant pathogen S. aureus.

First Name
Last Name
Email Address
Comments
Security code


Abstract View: 2801

Your browser does not support the canvas element.


PDF Download: 1549

Your browser does not support the canvas element.


Full Text View: 1109

Your browser does not support the canvas element.

Submitted: 06 Feb 2018
Revision: 19 Apr 2018
Accepted: 25 Apr 2018
ePublished: 01 May 2018
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)