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Bioimpacts. 2012;2(1): 47-53.
doi: 10.5681/bi.2012.006
PMID: 23678441
PMCID: PMC3648916
Scopus ID: 84862671491
  Abstract View: 1820
  PDF Download: 966

Original Research

Antioxidant, Total Phenol and Flavonoid Contents of Two Pedicularis L. Species from Eastern Azerbaijan, Iran

Laleh Khodaie 1,2, Sedigheh Bamdad 1, Abbas Delazar 1, Hossein Nazemiyeh 3*

1 Drug Applied Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Email: nazemiyehh@yahoo.com

Abstract

Introduction: Pedicularis sibthorpii and P. wilhelmsiana are endemic species mainly found in North-West of Iran. Plants of genus Pedicularis produce some important polyphenols and flavonoids. In the present work, total phenol and flavonoid contents of the mentioned species as well as their antioxidant capacity have been evaluated. Methods:  Methanol extract of samples was fractionated by SPE method using an ODS cartridge and their 1H-NMR spectra were recorded. Total phenols and flavonoids of methanol extracts were determined using Folin- Ciocalteu and aluminum chloride methods. For determining antioxidant activity of the extracts and fractions, bleaching of purple colour methanol solution of 1, 1-diphenylpycryl hydrazyl (DPPH) was measured by spectrophotometric assay. Results: Total phenols of Pedicularis sibthorpii and P. wilhelmsiana were in the range of 8-30 mg g-1 and 9-20 mg g-1, respectively. The 40% and 60% fractions of P.sibthorpii and the 20%, 40% and 60% fractions of P. wilhelmsiana showed higher amounts of phenolic compounds. The total flavonoid contents of P. sibthorpii and P. wilhelmsiana were in the range of 0-215 mg g-1 and 0-177 mg g-1, respectively, whereas the 40% and 60% fractions showed higher flavonoid amounts. Antioxidant activity of P. sibthorpii and P. wilhelmsiana were in the range of 0.01-0.7 mg mL-1 and 0.01-1.02 mg mL-1. In the same manner, the 20% and 40% fractions of P. sibthorpii and the 40% and 60% fractions of P. wilhelmsiana had lower RC50 than that of other fractions. Conclusion:  Fractions with lower RC50 had higher contents of phenolic and flavonoid compounds. The results of NMR spectra were parallel with these findings and show that it is worth to do phytochemical studies on P. sibthorpii and P. wilhelmsiana.
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Submitted: 13 Feb 2012
Revision: 06 Mar 2012
Accepted: 20 Mar 2012
ePublished: 24 Mar 2012
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