Bioimpacts. 2022;12: 33-42.
doi: 10.34172/bi.2021.22037
  Abstract View: 147
  PDF Download: 59
  Full Text View: 5

Original Research

Therapeutic effects of silver nanoparticle and L-carnitine on aerobic vaginitis in mice: an experimental study

Mozhgan Fatahi Dehpahni 1 ORCID logo, Khosrow Chehri 1* ORCID logo, Mehri Azadbakht 1 ORCID logo

1 Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran
*Corresponding Author: Corresponding author: Khosrow Chehri, Email: khchehri@gmail.com


Introduction: Aerobic vaginitis (AV) is a type of vaginal infection that occurs at the reproductive age of women. In this study, we aimed to study the possible anti-AV therapeutic effects of silver nanoparticles (AgNPs) and L-carnitine (LC) in the mouse model.
Methods: AV model was established by intra-vaginal inoculation of 108 CFU/mL Staphylococcus aureus and Escherichia coli (1:1) in adult NMRI mice. Susceptibilities of the bacteria were examined against AgNPs by inhibitory concentration (IC-50 and IC-90) and minimum biofilm inhibitory concentration (MBIC- 90) methods. The regimens therapy was intra-vaginal inoculation of AgNPs at MBIC- 90 and a daily injection of 250 mg/kg LC for two weeks. Mice were classified into healthy (control) and AV groups and then treated by LC, AgNPs, and AgNPs + LC. The vaginal smears were taken daily and tissue sections were prepared using the hematoxylin and eosin (H & E) method.
Results: Minimum inhibitory concentrations (MICs) of AgNPs for E. coli, S. aureus, and their mixture were 250, 125, and 500 ppm, and their MBIC-90% were 500, 250, and 1000 ppm, respectively. The estrus cycle of mice treated with co-administration of AgNPs and LC was similar to the control group (P < 0.05). The results of histology also showed that infected mice were treated with AgNPs and LC, simultaneously.
Conclusion: Single bacteria are more sensitive than their mixed model to these NPs. Co-administration of AgNPs as an antibacterial agent and LC as an antioxidant agent can treat AV in the infected mice.

Keywords: Aerobic vaginitis, Staphylococcus aureus, Escherichia coli, Metal nanoparticle, L-carnitine
First Name
Last Name
Email Address
Security code

Abstract View: 142

Your browser does not support the canvas element.

PDF Download: 59

Your browser does not support the canvas element.

Full Text View: 5

Your browser does not support the canvas element.

Submitted: 18 Feb 2020
Revision: 28 Oct 2020
Accepted: 14 Nov 2020
ePublished: 25 Oct 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)