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Bioimpacts. 2024;14(4): 28902.
doi: 10.34172/bi.2023.28902
  Abstract View: 205
  PDF Download: 163

Original Article

Design and implementation of a lab-on-a-chip for assisted reproductive technologies

Firooz Safaefar 1 ORCID logo, Javad Karamdel 2* ORCID logo, Hadi Veladi 1,3* ORCID logo, Masoud Maleki 1,4 ORCID logo

1 Department of Biomedical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad ‎University, Tehran, Iran
2 Department of Electrical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad ‎University, Tehran, Iran
3 Microsystem Fabrication Laboratory, Faculty of Electrical and Computer Engineering, University of Tabriz, Tabriz, ‎Iran
4 Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
*Corresponding Authors: Javad Karamdel, Email: j-karamdel@azad.ac.ir; Hadi Veladi, Email: veladi@tabrizu.ac.ir

Abstract

Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods.
Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfluidic chip was modeled and simulated using COMSOL Multiphysics® 5.2 software to optimize and enhance its design and performance. The microfluidic chip was fabricated using conventional injection molding techniques on a polydimethylsiloxane substrate by employing soft lithography methods.
Results: A hydrostatic force was applied to guide the oocyte through predetermined pathways to eliminate the cumulus cells surrounding the oocyte. The oocyte was subsequently confined within the designated trap region by utilizing hydraulic resistance along the paths and immobilized by applying vacuum force.
Conclusion: The application of this chip necessitates a lower level of operator expertise compared to enzymatic and mechanical techniques. Moreover, it is feasible to continuously monitor the oocyte's state throughout the procedure. There is a reduced need for cultural media compared to more standard approaches.
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Submitted: 08 May 2023
Revision: 29 Oct 2023
Accepted: 14 Nov 2023
ePublished: 10 Dec 2023
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