Bioimpacts. 2024;14(3): 29981.
doi: 10.34172/bi.2023.29981
  Abstract View: 143
  PDF Download: 80

Original Article

Podocyte-specific proteins in urinary extracellular vesicles of patients with IgA nephropathy: Vasorin and ceruloplasmin

Negin Farzamikia 1,2 ORCID logo, Seyyedeh Mina Hejazian 1, Soroush Mostafavi 3, Behzad Baradaran 4, Sepideh Zununi Vahed 1* ORCID logo, Mohammadreza Ardalan 1* ORCID logo

1 Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Cardiology, Hazrat-e-Rasool General Hospital, Iran University of Medical ‎Sciences, Tehran, Iran
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Authors: Sepideh Zununi Vahed, Email: sepide.zununi@gmail.com; Mohammadreza Ardalan, ardalanm@tbzmed.ac.ir, Email: ardalan34@yahoo.com


Introduction: Urinary extracellular vesicles (uEVs) can be considered biomarkers of kidney diseases. EVs derived from podocytes may reflect podocyte damage in different glomerular diseases. IgA nephropathy (IgAN) is one of the most common forms of glomerulonephritis (GN) characterized by proteinuria and hematuria. This study aimed to analyze the uEVs of IgAN patients to understand the pathophysiological processes of the disease at the protein level.
Methods: Patients with GN [biopsy-proven IgAN (n = 16) and membranous glomerulonephritis (MGN, n = 16)], and healthy controls (n = 16) were included in this study. The uEVs were extracted, characterized, and analyzed to evaluate the protein levels of candidate markers of IgAN, including vasorin precursor, aminopeptidase N, and ceruloplasmin by western-blot analysis.
Results: Higher levels of both podocytes and EVs-related proteins were observed in the pooled urine samples of GN patients compared to the healthy controls. In IgAN patients, uEV-protein levels of vasorin were statistically lower while levels of ceruloplasmin were significantly higher compared to MGN (P = 0.002, P = 0.06) and healthy controls, respectively (P = 0.020, P= 0.001).
Conclusion: Different levels of the studied proteins in uEVs may indicate podocyte injury and represent a direct association with the pathology of IgAN and MGN.
First Name
Last Name
Email Address
Security code

Abstract View: 144

Your browser does not support the canvas element.

PDF Download: 80

Your browser does not support the canvas element.

Submitted: 05 Jul 2023
Revision: 08 Oct 2023
Accepted: 09 Oct 2023
ePublished: 31 Oct 2023
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)