Aakriti Tyagi
1 
, Disha Mittal
1,2 , S. Bhanoth
3, Ankita Leekha
1,4, Anita K. Verma
1,5* 1 Nanobiotech lab, Kirori Mal College, University of Delhi, 110007, Delhi, India
2 School of Biological and Life Sciences, Greater Noida, Uttar Pradesh, India
3 Department of Applied Chemistry, Defence Institute of Advanced Technology,Ministry of Defence Govt. of India
4 Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA
5 Fellow, Delhi School of Public Health, Institution of Eminence, University of Delhi, 110007, Delhi, India
Abstract
Introduction: Quantum dots (QDs) are semiconductor nanocrystals with inherent fluorescence having several advantages over traditional fluorescent probes including their small size (5–10 nm), tunable excitation and emission spectra, ease of surface functionalization, and robust photostability that makes them ideal candidates for in vivo imaging. The behavior of QDs is highly dependent on the surface functionality. In vivo toxicity of QDs in biological systems is the major limitation hindering their translation to clinics.
Methods: In the present study, cytotoxicity of water soluble ZnSe/CdS core shell QDs capped with oleic acid was evaluated against human hepatocellular carcinoma cell line-Hep3B, Human Embryonic Kidney cell line-HEK 293 and Ehlrich Ascitic cells-EAC. To assess its in vivo therapeutic efficacy, the initial animal toxicity studies of OA capped ZnSe/ CdS QDs were done in Balb/c mice. Serum stability, pharmacokinetics, biodistribution and γ-scintigraphic imaging were observed in mice after intravenous (i.v) injection of QDs at a dose of 10 nM/kg/200 µL/mice up to 28 days.
Results: IC50 of OA capped QDs against Hep3B was 29.85 µg/mL at 24 hours. QDs toxicity was primarily due to the generation of reactive oxygen species as observed by LDH release in Hep3B cells. Negligible haemolysis indicated that OA capped QDs were biocompatible. OA capped QDs mainly accumulated in the liver and spleen with no retention in kidneys.
Conclusion: OA capped ZnSe/ CdS QDs exhibited enhanced anti-cancer efficacy against Hep3B and EAC cell line. Further, minimum accumulation and retention were observed in vital organs in Balb/c mice protecting them from potential adverse side effects, underlining their potential for biomedical applications.