Logo-bi
Bioimpacts. 2018;8(1): 39-52.
doi: 10.15171/bi.2018.06
PMID: 29713601
PMCID: PMC5915707
Scopus ID: 85044310762
  Abstract View: 2380
  PDF Download: 1473
  Full Text View: 1232

Original Research

A novel B- and helper T-cell epitopes-based prophylactic vaccine against Echinococcus granulosus

Mohammad Mostafa Pourseif, Gholamali Moghaddam*, Hossein Daghighkia, Ahmad Nematollahi, Yadollah Omidi* ORCID logo

1 Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Pathobiology, Veterinary Collage, University of Tabriz, Tabriz, Iran
4 Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Authors: Email: ghmoghaddam@tabrizu.ac.ir; Email: yadollah.omidi@gmail.com

Abstract

Introduction: In this study, we targeted the worm stage of Echinococcus granulosus to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite.

Methods: The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep’s liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. For the vaccine designing, several in silico steps were undertaken. Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. The protein modeling accuracy was then analyzed via various validation assays. Potential transmembrane helix, signal peptide, post-translational modifications and allergenicity of Eg14-3-3 were evaluated as the preliminary measures of B-cell epitopes (BEs) prediction. Having used many web-servers, a well-designed process was carried out for improved prediction of BEs. High ranked linear and conformational BEs were utilized for engineering the final vaccine construct. Possible T-helper epitopes (TEs) were identified by the molecular docking between 13-mer fragments of the Eg14-3-3 Ag and two high frequent dog class II MHC alleles (i.e., DLA-DRB1*01101 and DRB1*01501). The epitopes coverage was evaluated by Shannon’s variability plot.

Results: The final designed construct was analyzed based on different physicochemical properties, which was then codon optimized for high-level expression in Escherichia coli k12. This minigene construct is the first dog-specific epitopic vaccine construct that is established based on TEs with high-binding affinity to canine MHC alleles.

Conclusion: This in silico study is the first part of a multi-antigenic vaccine designing work that represents as a novel dog-specific vaccine against E. granulosus. Here, we present key data on the step-by-step methodologies used for designing this de novo vaccine, which is under comprehensive in vivo investigations.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 2379

Your browser does not support the canvas element.


PDF Download: 1473

Your browser does not support the canvas element.


Full Text View: 1232

Your browser does not support the canvas element.

Submitted: 06 Nov 2017
Revision: 02 Dec 2017
Accepted: 03 Dec 2017
ePublished: 20 Dec 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)