Bioimpacts. 2019;9(1):25-36.
doi: 10.15171/bi.2019.04
PMID: 30788257
PMCID: PMC6378099
Scopus id: 85060479725
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Original Research

In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent

Cécilia Delattre 1 * , Diego Velazquez 1, Caroline Roques 1,2, Graciela Pavon-Djavid 1,2, Véronique Ollivier 1,2, Anna Lokajczyk 3,4, Thierry Avramoglou 1,2, Virginie Gueguen 1,2, Liliane Louedec 1,2, Giuseppina Caligiuri 1,2, Martine Jandrot-Perrus 1,2, Catherine Boisson-Vidal 3,4, Didier Letourneur 1,2, Anne Meddahi-Pelle 1,2

1 INSERM, UMR_S1148, Laboratory for Vascular Translational Sciences, Hôpital Bichat
2 Université Paris 13, Sorbonne Paris Cité, France
3 Inserm UMR_S1140, Paris France
4 Université Paris Descartes, Sorbonne Paris Cité, France

Abstract

Introduction: The major complications of stent implantation are restenosis and late stent thrombosis. PBMA polymers are used for stent coating because of their mechanical properties. We previously synthesized and characterized Dextrangraft-polybutylmethacrylate copolymer (Dex-PBMA) as a potential stent coating. In this study, we evaluated the haemocompatibility and biocompatibility properties of Dex-PBMA in vitro and in vivo.

Methods: Here, we investigated: (1) the effectiveness of polymer coating under physiological conditions and its ability to release Tacrolimus®, (2) the capacity of Dex-PBMA to inhibit Staphylococcus aureus adhesion, (3) the thrombin generation and the human platelet adhesion in static and dynamic conditions, (4) the biocompatibility properties in vitro on human endothelial colony forming cells ( ECFC) and on mesenchymal stem cells (MSC) and in vivo in rat models, and (5) we implanted Dex-PBMA and Dex-PBMATAC coated stents in neointimal hyperplasia restenosis rabbit model.

Results: Dex-PBMA coating efficiently prevented bacterial adhesion and release Tacrolimus®. Dex-PBMA exhibit haemocompatibility properties under flow and ECFC and MSC compatibility. In vivo, no pathological foreign body reaction was observed neither after intramuscular nor intravascular aortic implantation. After Dex-PBMA and Dex-PBMATAC coated stents 30 days implantation in a restenosis rabbit model, an endothelial cell coverage was observed and the lumen patency was preserved.

Conclusion: Based on our findings, Dex-PBMA exhibited vascular compatibility and can potentially be used as a coating for metallic coronary stents.

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Submitted: 29 May 2018
Revised: 17 Sep 2018
Accepted: 24 Sep 2018
First published online: 02 Oct 2018
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