Bioimpacts. 2019;9(2):71-78.
doi: 10.15171/bi.2019.10
  Abstract View: 363
  PDF Download: 38

Original Research

SPR signals enhancement by gold nanorods for cell surface marker detection

Farzaneh Fathi 1,2 * ORCiD, Roghayeh Jalili 3, Mohammad Amjadi 3, Mohammad-Reza Rashidi 1,4 *

1 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
4 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Introduction: The detection of micrometer-sized particles like cells is limited by surface plasmon resonance (SPR) biosensors because of having a depth of evanescent wave <500 nm. In this study, for the first time, we exhibited the use of streptavidin-functionalized gold nanorods (GNRs) as intensification labels for detection of cell surface markers in SPR-based biosensors.
Methods: The GNRs (ʎ max: 735 nm) were modified with streptavidin using EDC/NHS coupling method and human umbilical vein endothelial cells (HUVECs) were selected as the cell model for detecting VE-cadherin on cell surface using real-time SPR device in the 785 nm wavelength of the laser source.
Results: The investigations revealed that the plasmonic field extension produced from the gold layer and GNRs resulted in multiple enhancement of SPR signals when the wavelength of laser source in SPR instrument was matched with the wavelength of maximum absorbance in GNRs. Moreover, the results showed that the growth of ∆RU value in specific and non-specific bindings for various cell number injections were produced with increasing the cell number.
Conclusion: The results displayed that cell detection can be performed in real- time form without any need to a time-consuming process used in conventional methods like immunocytochemistry, flow cytometry, and western blotting.
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Submitted: 06 Aug 2018
Revised: 06 Oct 2018
Accepted: 07 Oct 2018
First published online: 20 Oct 2018
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