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BioImpacts. 2021;11(2): 119-127.
doi: 10.34172/bi.2021.19
PMID: 33842282
PMCID: PMC8022237
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Original Research

In silico approach towards the identification of potential inhibitors from Curcuma amada Roxb against H. pylori: ADMET screening and molecular docking studies

G Divyashri 1* ORCID logo, T P Krishna Murthy 1, Subramaniam Sundareshan 1, Pavan Kamath 1, Manikanta Murahari 2,3*, G R Saraswathy 2,4, Bindu Sadanandan 1

1 Department of Biotechnology, M S Ramaiah Institute of Technology, Bangalore, Karnataka, India
2 Pharmacological Modelling and Simulation Centre, M.S. Ramaiah University of Applied Sciences, Bangalore, Karnataka, India
3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, Bangalore, Karnataka, India
4 Department of Pharmacy Practice, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, Bangalore, Karnataka, India
Corresponding authors: Divyashri G, Email: gdivyashri@gmail.com; Manikanta Murahari, Email: manikanta.murahari@gmail.com gdivyashri@gmail.com

Abstract

Introduction: The present study attempts to identify potential targets of H. pylori for novel inhibitors from therapeutic herb, mango ginger (Curcuma amada Roxb.).
Methods: Crystal structure of all the selected drug targets obtained from Protein Data Bank (PDB) were subjected to molecular docking against a total of 130 compounds (found to have biological activity against H. pylori) were retrieved from public databases. Compounds with good binding affinity were selected for Prime MM-GBSA rescoring and molecular dynamics (MD) simulation. Final list of compounds were taken for ADMET predictions.
Results: Based on binding affinity denoted by glide score and ligand efficiency, mango ginger compounds were found selective to shikimate kinase and type II dehydroquinase through hydrogen bonding and salt bridge interactions. Stability of the interactions and free energy calculations by Prime MM-GBSA results confirmed the affinity of mango ginger compounds towards both shikimate kinase and type II dehydroquinase. From the above results, 15 compounds were calculated for ADMET parameters, Lipinski’s rule of five, and the results were found promising without any limitations. MD simulations identified gentisic acid as hit compound for shikimate kinase of H. pylori.
Conclusion: Current study could identify the in silico potential of mango ginger compounds against shikimate kinase and type II dehydroquinase targets for H. pylori infections and are suitable for in vitro and in vivo evaluation.
Keywords: Mango ginger, H. pylori, Gentisic acid, Molecular dynamics, Docking, Lipinski’s rule of five
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Submitted: 29 Jan 2020
Revision: 05 Mar 2020
Accepted: 16 Mar 2020
ePublished: 24 Mar 2020
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