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Bioimpacts. 2016;6(1): 25-31. doi: 10.15171/bi.2016.04
PMID: 27340621        PMCID: PMC4916548

Original Research

A microRNA isolation method from clinical samples

Sepideh Zununi Vahed 1,2,3, Abolfazl Barzegari 1,3, Yalda Rahbar Saadat 1, Somayeh Mohammadi 4, Nasser Samadi 2,3 *

Cited by CrossRef: 25

1- Ozkaraca M, Ozdemir S, Comakli S, Timurkan M. Roles of apoptosis and autophagy in natural rabies infections. Vet Med. 2022;67(1):1 [Crossref]
2- Ashirbekov Y, Abaildayev A, Omarbayeva N, Botbayev D, Belkozhayev A, Askandirova A, Neupokoyeva A, Utegenova G, Sharipov K, Aitkhozhina N. Combination of circulating miR-145-5p/miR-191-5p as biomarker for breast cancer detection. 2020;8:e10494 [Crossref]
3- Ban E, Song E. Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR. Genes. 2022;13(2):328 [Crossref]
4- Abbasi A, Rad A, Maleki L, Kafil H, Baghbanzadeh A. Antigenotoxicity and Cytotoxic Potentials of Cell-Free Supernatants Derived from Saccharomyces cerevisiae var. boulardii on HT-29 Human Colon Cancer Cell Lines. Probiotics & Antimicro Prot. 2023;15(6):1583 [Crossref]
5- Çomaklı S, Özdemir S, Güloğlu M. Chrysin attenuates paclitaxel-induced hepatorenal toxicity in rats by suppressing oxidative damage, inflammation, and apoptosis. Life Sciences. 2023;332:122096 [Crossref]
6- Garrison Z, Hornick N, Cheng J, Kulkarni R. Circulating biomarkers of response to immunotherapy and immune-related adverse events. Expert Review of Molecular Diagnostics. 2022;22(9):855 [Crossref]
7- Padda J, Khalid K, Khedr A, Patel V, Al-Ewaidat O, Tasnim F, Padda S, Cooper A, Jean-Charles G. Exosome-Derived microRNA: Efficacy in Cancer. 2021; [Crossref]
8- Mirzajani E, Vahidi S, Norollahi S, Samadani A. Novel Biomarkers of microRNAs in Gastric Cancer: An Overview from Diagnosis to Treatment. MIRNA. 2022;11(1):12 [Crossref]
9- Sambrani R, Abdolalizadeh J, Kohan L, Jafari B. Saccharomyces cerevisiae inhibits growth and metastasis and stimulates apoptosis in HT-29 colorectal cancer cell line. Comp Clin Pathol. 2019;28(4):985 [Crossref]
10- Pitou M, Papi R, Tzavellas A, Choli-Papadopoulou T. ssDNA-Modified Gold Nanoparticles as a Tool to Detect miRNA Biomarkers in Osteoarthritis. ACS Omega. 2023;8(8):7529 [Crossref]
11- Meng J, Ding J, Wang W, Gu B, Zhou F, Wu D, Fu X, Liu J. Reversal of gentamicin sulfate resistance in avian pathogenic Escherichia coli by matrine combined with berberine hydrochloride. Arch Microbiol. 2024;206(7) [Crossref]
12- Anobile D, Montenovo G, Pecoraro C, Franczak M, Ait Iddouch W, Peters G, Riganti C, Giovannetti E. Splicing deregulation, microRNA and notch aberrations: fighting the three-headed dog to overcome drug resistance in malignant mesothelioma. Expert Review of Clinical Pharmacology. 2022;15(3):305 [Crossref]
13- Das K, Paul S, Singh A, Ghosh A, Roy A, Ansari S, Prasad R, Mukherjee A, Sen P. Triple-negative breast cancer-derived microvesicles transfer microRNA221 to the recipient cells and thereby promote epithelial-to-mesenchymal transition. Journal of Biological Chemistry. 2019;294(37):13681 [Crossref]
14- Duguet T, Soichot J, Kuzyakiv R, Malmström L, Tritten L. Extracellular Vesicle-Contained microRNA of C. elegans as a Tool to Decipher the Molecular Basis of Nematode Parasitism. Front Cell Infect Microbiol. 2020;10 [Crossref]
15- Zununi Vahed S, Barzegari A, Rahbar Saadat Y, Goreyshi A, Omidi Y. Leuconostoc mesenteroides-derived anticancer pharmaceuticals hinder inflammation and cell survival in colon cancer cells by modulating NF-κB/AKT/PTEN/MAPK pathways. Biomedicine & Pharmacotherapy. 2017;94:1094 [Crossref]
16- Bravo-Vázquez L, Frías-Reid N, Ramos-Delgado A, Osorio-Pérez S, Zlotnik-Chávez H, Pathak S, Banerjee A, Bandyopadhyay A, Duttaroy A, Paul S. MicroRNAs and long non-coding RNAs in pancreatic cancer: From epigenetics to potential clinical applications. Translational Oncology. 2023;27:101579 [Crossref]
17- Khnouf R, Han C, Munro S. Isolation of enriched small RNA from cell‐lysate using on‐chip isotachophoresis. Electrophoresis. 2019;40(23-24):3140 [Crossref]
18- Whitfield R, Anastasaki A, Truong N, Cook A, Omedes-Pujol M, Loczenski Rose V, Nguyen T, Burns J, Perrier S, Davis T, Haddleton D. Efficient Binding, Protection, and Self-Release of dsRNA in Soil by Linear and Star Cationic Polymers. ACS Macro Lett. 2018;7(8):909 [Crossref]
19- Li T, Ren J, Ma J, Wu J, Zhang R, Yuan H, Han X. LINC00702/miR-4652-3p/ZEB1 axis promotes the progression of malignant meningioma through activating Wnt/β-catenin pathway. Biomedicine & Pharmacotherapy. 2019;113:108718 [Crossref]
20- Rahbar Saadat Y, Pourseif M, Zununi Vahed S, Barzegari A, Omidi Y, Barar J. Modulatory Role of Vaginal-Isolated Lactococcus lactis on the Expression of miR-21, miR-200b, and TLR-4 in CAOV-4 Cells and In Silico Revalidation. Probiotics & Antimicro Prot. 2020;12(3):1083 [Crossref]
21- Aamazadeh F, Barar J, Rahbar Saadat Y, Ostadrahimi A. In vitro evaluation of cytotoxic and apoptotic activities of ethanolic extract of sweet apricot kernel on PANC-1 pancreatic cancer cells. NFS. 2022;52(1):12 [Crossref]
22- Jacob G, Shimomura K, Hanai H, Akimori T, Ohori T, Tsujii A, Moriguchi Y, Nakamura N. Therapeutic potential of microRNA in meniscal repair and regeneration. The Knee. 2025;55:18 [Crossref]
23- Zununi Vahed S, Omidi Y, Ardalan M, Samadi N. Dysregulation of urinary miR-21 and miR-200b associated with interstitial fibrosis and tubular atrophy (IFTA) in renal transplant recipients. Clinical Biochemistry. 2017;50(1-2):32 [Crossref]

As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
Acceptance rate (2024): 20% 
Publication fee: Free of charge

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