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Bioimpacts. 2017;7(3): 199-206. doi: 10.15171/bi.2017.23
PMID: 29159147        PMCID: PMC5684511

Original Research

Osmotic conditions could promote scFv antibody production in the Escherichia coli HB2151

Ali Mesgari-Shadi 1,2, Mohammad Hossein Sarrafzadeh 1 *

Cited by CrossRef: 11


1- Ghamghami E, Abri Aghdam M, Tohidkia M, Ahmadikhah A, Khanmohammadi M, Mehdipour T, Mokhtarzadeh A, Baradaran B. Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies. AMB Expr. 2020;10(1) [Crossref]
2- Tahmasebi A, Ghaffari S, Sarrafzadeh M. Techno-economic comparison of two hydroxyl and sulfate radicals based advanced oxidation process for enhancing biodegradability of pulp and paper mill wastewater. Chemical Engineering Science. 2024;293:120095 [Crossref]
3- Pasupuleti R, Rosato F, Kolanovic D, Makshakova O, Römer W, Wiltschi B. Genetic code expansion in E. coli enables production of a functional ‘ready-to-click’ T cell receptor-specific scFv. New Biotechnology. 2023;76:127 [Crossref]
4- Eskafi A, Bagheri K, Behdani M, Yamabhai M, Shahbazzadeh D, Kazemi-Lomedasht F. Development and characterization of human single chain antibody against Iranian Macrovipera lebetina snake venom. Toxicon. 2021;197:106 [Crossref]
5- Mesgari-Shadi A, Sarrafzadeh M, Divband B, Barar J, Omidi Y. Batch adsorption/desorption for purification of scFv antibodies using nanozeolite microspheres. Microporous and Mesoporous Materials. 2018;264:167 [Crossref]
6- Mobayed F, Nunes J, Gennari A, de Andrade B, Ferreira M, Pauli P, Renard G, Chies J, Volpato G, Volken de Souza C. Effect of by-products from the dairy industry as alternative inducers of recombinant β-galactosidase expression. Biotechnol Lett. 2021;43(3):589 [Crossref]
7- Xu C, Yu M, Xie Y, Zhong J, Chen W, Lin M, Hu X, Shen Y. Screening and identification of vancomycin anti-idiotypic antibodies for against Staphylococcus aureus from a human phage display domain antibody library. Immunology Letters. 2022;246:1 [Crossref]
8- Jafari B, Hamzeh-Mivehroud M, Morris M, Dastmalchi S. Exploitation of phage display for the development of anti-cancer agents targeting fibroblast growth factor signaling pathways: New strategies to tackle an old challenge. Cytokine & Growth Factor Reviews. 2019;46:54 [Crossref]
9- Xu C, Liu X, Liu Y, Zhang X, Zhang C, Li J, Liu X. High sensitive single chain variable fragment screening from a microcystin-LR immunized mouse phage antibody library and its application in immunoassay. Talanta. 2019;197:397 [Crossref]
10- Petrus M, Kiefer L, Puri P, Heemskerk E, Seaman M, Barouch D, Arias S, van Wezel G, Havenga M. A microbial expression system for high-level production of scFv HIV-neutralizing antibody fragments in Escherichia coli. Appl Microbiol Biotechnol. 2019;103(21-22):8875 [Crossref]
11- Mesgari-Shadi A, Sarrafzadeh M, Barar J, Omidi Y. Cost-effective batch production process of scFv antibody in Escherichia coli. HAB. 2018;26(3):149 [Crossref]


As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
Acceptance rate: 24% 
Publication fee: Free of charge