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Bioimpacts. 2019;9(1): 15-23. doi: 10.15171/bi.2019.03
PMID: 30788256        PMCID: PMC6378094

Original Research

Highly efficient novel recombinant L-asparaginase with no glutaminase activity from a new halo-thermotolerant Bacillus strain

Azam Safary 1 ORCID, Rezvan Moniri 2,3, Maryam Hamzeh-Mivehroud 4,5, Siavoush Dastmalchi 4,5,6 *

Cited by CrossRef: 29

1- Safary A, Akbarzadeh Khiavi M, Omidi Y, Rafi M. Targeted enzyme delivery systems in lysosomal disorders: an innovative form of therapy for mucopolysaccharidosis. Cell Mol Life Sci. 2019;76(17):3363 [Crossref]
2- Farahat M, Amr D, Galal A. Molecular cloning, structural modeling and characterization of a novel glutaminase-free L-asparaginase from Cobetia amphilecti AMI6. International Journal of Biological Macromolecules. 2020;143:685 [Crossref]
3- Sengupta S, Biswas M, Gandhi K, Gupta S, Gera P, Gota V, Sonawane A. Preclinical evaluation of engineered L-asparaginase variants to improve the treatment of Acute Lymphoblastic Leukemia. Translational Oncology. 2024;43:101909 [Crossref]
4- Kumar S, Darnal S, Patial V, Kumar V, Kumar V, Kumar S, Singh D. Molecular cloning, characterization, and in-silico analysis of l-asparaginase from Himalayan Pseudomonas sp. PCH44. 3 Biotech. 2022;12(8) [Crossref]
5- Barros T, Brumano L, Freitas M, Pessoa A, Parachin N, Magalhães P. Development of Processes for Recombinant L-Asparaginase II Production by Escherichia coli Bl21 (De3): From Shaker to Bioreactors. Pharmaceutics. 2020;13(1):14 [Crossref]
6- Pourseif M, Moghaddam G, Nematollahi A, Khordadmehr M, Naghili B, Dehghani J, Omidi Y. Vaccination with rEGVac elicits immunoprotection against different stages of Echinococcus granulosus life cycle: A pilot study. Acta Tropica. 2021;218:105883 [Crossref]
7- Dastmalchi M, Alizadeh M, Jamshidi-Kandjan O, Rezazadeh H, Hamzeh-Mivehroud M, Farajollahi M, Dastmalchi S. Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli. Adv Pharm Bull. 2023;13(4):827 [Crossref]
8- Olfati A, Akbarzadeh-Khiavi M, Safary A, Pourseif M, Adibkia K. Biological characterisation and computational conformation dynamics of putative L-glutaminase YLaM identified from Bacillus licheniformis . Molecular Simulation. 2024;50(16):1367 [Crossref]
9- Akbarzadeh Khiavi M, Safary A, Somi M. Recent advances in targeted therapy of colorectal cancer: impacts of monoclonal antibodies nanoconjugates. Bioimpacts. 2019;9(3):123 [Crossref]
10- Fathi M, Safary A, Barar J. Therapeutic impacts of enzyme-responsive smart nanobiosystems. Bioimpacts. 2019;10(1):1 [Crossref]
11- Dumina M, Zhgun A, Pokrovskaya M, Aleksandrova S, Zhdanov D, Sokolov N, El’darov M. A Novel L-Asparaginase from Hyperthermophilic Archaeon Thermococcus sibiricus: Heterologous Expression and Characterization for Biotechnology Application. IJMS. 2021;22(18):9894 [Crossref]
12- Akbarzadeh Khiavi M, Safary A, Aghanejad A, Barar J, Rasta S, Golchin A, Omidi Y, Somi M. Enzyme-conjugated gold nanoparticles for combined enzyme and photothermal therapy of colon cancer cells. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2019;572:333 [Crossref]
13- Dumina M, Zhgun A, Pokrovskay M, Aleksandrova S, Zhdanov D, Sokolov N, El’darov M. Comparison of Enzymatic Activity of Novel Recombinant L-asparaginases of Extremophiles. Appl Biochem Microbiol. 2021;57(5):594 [Crossref]
14- Aslzad S, Heydari P, Abdolahinia E, Amiryaghoubi N, Safary A, Fathi M, Erfan-Niya H. Chitosan/gelatin hybrid nanogel containing doxorubicin as enzyme-responsive drug delivery system for breast cancer treatment. Colloid Polym Sci. 2023;301(3):273 [Crossref]
15- Dumina M, Eldarov M, Zdanov D, Sokolov N. L-Asparaginases of Extremophilic Microorganisms in Biomedicine. Biochem Moscow Suppl Ser B. 2020;14(4):277 [Crossref]
16- Dantas R, Caetano L, Torres A, Alves M, Silva E, Teixeira L, Teixeira D, de Azevedo Moreira R, Fonseca M, Gaudêncio Neto S, Martins L, Furtado G, Tavares K. Expression of a recombinant bacterial l-asparaginase in human cells. BMC Res Notes. 2019;12(1) [Crossref]
17- Shanehbandi D, Asadi M, Seyedrezazadeh E, Zafari V, Shekari N, Akbari M, Rahbarnia L, Zarredar H. MicroRNA-Based Biomarkers in Lung Cancer: Recent Advances and Potential Applications. CMM. 2023;23(7):648 [Crossref]
18- Roudi R, Barodabi M, Madjd Z, Roviello G, Corona S, Panahi M. Expression patterns and clinical significance of the potential cancer stem cell markers OCT4 and NANOG in colorectal cancer patients. Molecular & Cellular Oncology. 2020;7(5) [Crossref]
19- Akbarzadeh Khiavi M, Safary A, Barar J, Farzi-Khajeh H, Barzegari A, Mousavi R, Somi M, Omidi Y. PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells. Bioimpacts. 2019;10(1):27 [Crossref]
20- Simay S, Akbarzadeh-Khiavi M, Pourseif M, Barar J, Safary A, Omidi Y. Recombinant production and characterization of L-glutaminase (glsA) as a promiscuity therapeutic enzyme. Appl Microbiol Biotechnol. 2022;106(17):5511 [Crossref]
21- Lassaletta Á, Gutiérrez F. Asparaginase activity monitoring in pediatric acute lymphoblastic leukemia: A cross‐sectional nationwide study in Spain. Cancer Reports. 2023;6(2) [Crossref]
22- Al Yousef S. Fusarium sp. l-asparaginases: purification, characterization, and potential assessment as an antileukemic chemotherapeutic agent. Environ Sci Pollut Res. 2022;29(8):11243 [Crossref]
23- Shishparenok A, Gladilina Y, Zhdanov D. Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production. IJMS. 2023;24(20):15220 [Crossref]
24- Jia R, Wan X, Geng X, Xue D, Xie Z, Chen C. Microbial L-asparaginase for Application in Acrylamide Mitigation from Food: Current Research Status and Future Perspectives. Microorganisms. 2021;9(8):1659 [Crossref]
25- Tsegaye K, Tsehai B, Getie B. Desirable L-asparaginases for treating cancer and current research trends. Front Microbiol. 2024;15 [Crossref]
26- Castro D, Marques A, Almeida M, de Paiva G, Bento H, Pedrolli D, Freire M, Tavares A, Santos-Ebinuma V. L-asparaginase production review: bioprocess design and biochemical characteristics. Appl Microbiol Biotechnol. 2021;105(11):4515 [Crossref]
27- Dumina M, Eldarov M, Zdanov D, Sokolov N. L-asparaginases of extremophilic microorganisms in biomedicine. BIOMED KHIM. 2020;66(2):105 [Crossref]
28- Hadi W, Edwin B, Nair A. Unlocking the Potential of Marine Asparaginase Sources. Thalassas. 2024;40(1):147 [Crossref]

As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
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