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BioImpacts. 2021;11(4): 245-252. doi: 10.34172/bi.2021.32
PMID: 34631486        PMCID: PMC8494254

Original Research

Cell cycle arrest and anti-cancer potential of probiotic Lactobacillus rhamnosus against HT-29 cancer cells

Najme Dehghani 1 ORCID, Farzaneh Tafvizi 1 * ORCID, Parvaneh Jafari 2

Cited by CrossRef: 40

1- Salemi R, Vivarelli S, Ricci D, Scillato M, Santagati M, Gattuso G, Falzone L, Libra M. Lactobacillus rhamnosus GG cell-free supernatant as a novel anti-cancer adjuvant. J Transl Med. 2023;21(1) [Crossref]
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10- Haji Mehdi Nouri Z, Tafvizi F, Amini K, Khandandezfoli N, Kheirkhah B. Antiproliferative potential of Lactobacillus casei supernatant against MCF-7 breast cancer and HT-29 colorectal cancer cell lines. gebsj. 2023;12(2):216 [Crossref]
11- Wang S, Shan Y, Zhang S, Zhang L, Jiao Y, Xue D, Zhang L, Yi H. Lactobacillus paracasei subsp. paracasei X12 Strain Induces Apoptosis in HT-29 Cells through Activation of the Mitochondrial Pathway. Nutrients. 2023;15(9):2123 [Crossref]
12- Nowak A, Zakłos-Szyda M, Rosicka-Kaczmarek J, Motyl I. Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study. Cancers. 2022;14(7):1853 [Crossref]
13- Meng Z, He Q, Mu L, Feng J, Zhang F, Wu J, Zhou L, Hu Q, Tang X, Li Y. Pullulan-spermine enhance the tolerance of probiotics and immune stimulation of macrophages. International Journal of Biological Macromolecules. 2025;287:138417 [Crossref]
14- Di Luccia B, Acampora V, Saggese A, Calabrò V, Vivo M, Angrisano T, Baccigalupi L, Ricca E, Pollice A. Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183. Sci Rep. 2022;12(1) [Crossref]
15- Haji Mehdi Nouri Z, Tafvizi F, Amini K, Khandandezfully N, Kheirkhah B. Enhanced Induction of Apoptosis and Cell Cycle Arrest in MCF-7 Breast Cancer and HT-29 Colon Cancer Cell Lines via Low-Dose Biosynthesis of Selenium Nanoparticles Utilizing Lactobacillus casei. Biol Trace Elem Res. 2024;202(3):1288 [Crossref]
16- Dolati M, Tafvizi F, Salehipour M, Komeili Movahed T, Jafari P. Antiproliferative Potential of Bacillus coagulans Supernatant on SKBR3 Breast Cancer Cell Line. Iran J Med Microbiol. 2022;16(5):383 [Crossref]
17- Nama A, Sandeepa G, Buddolla V, Mastan A. Advances in understanding therapeutic mechanisms of probiotics in cancer management, with special emphasis on breast cancer: A comprehensive review. European Journal of Pharmacology. 2025;995:177410 [Crossref]
18- Kim S, Rahim M, Tajdozian H, Barman I, Park H, Yoon Y, Jo S, Lee S, Shuvo M, Bae S, Lee H, Ju S, Park C, Kim H, Han J, Kim J, Yoon S, Kim J, Choi Y, Lee S, Seo H, Song H. Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index. Cells. 2024;13(23):2029 [Crossref]
19- Su H, Cheung H, Lau H, Chen H, Zhang X, Qin N, Wang Y, Chan M, Wu W, Chen H. Crosstalk between gut microbiota and RNA N6-methyladenosine modification in cancer. 2023;47(4) [Crossref]
20- Panebianco C, Pisati F, Villani A, Andolfo A, Ulaszewska M, Bellini E, Ferro C, Lombardi R, Orsenigo F, Latiano T, Belmonte B, Tripodo C, Perri F, Pazienza V. Counteracting gemcitabine+nab-paclitaxel induced dysbiosis in KRAS wild type and KRASG12D mutated pancreatic cancer in vivo model. Cell Death Discov. 2023;9(1) [Crossref]
21- Chekhun V, Lykhova O, Bezdieniezhnykh N, Kukurudza D, Lupan V. THE ROLE OF LACTOBACILLUS RHAMNOSUS GG AS A MODIFIER OF THE HUMAN BREAST CANCER CELLS PROLIFERATION AND APOPTOTIC PROGRAM. Onkologiâ. 2023;25(1):16 [Crossref]
22- Ayed L, M’hir S, Nuzzolese D, Di Cagno R, Filannino P. Harnessing the Health and Techno-Functional Potential of Lactic Acid Bacteria: A Comprehensive Review. Foods. 2024;13(10):1538 [Crossref]
23- Anwer M, Wei M. Harnessing the power of probiotic strains in functional foods: nutritive, therapeutic, and next-generation challenges. Food Sci Biotechnol. 2024;33(9):2081 [Crossref]
24- Budu O, Banciu C, Soica C, Lighezan D, Milan A, Prodea A, Mioc A, Mioc M, Mardale G, Sima L. Lacticaseibacillus rhamnosus—A Promising Tool for Colorectal Cancer Treatment. Processes. 2023;11(3):781 [Crossref]
25- B S, C V, S K, B S, M I. Advancing Fermented Food Products: Exploring Bioprocess Technologies and Overcoming Challenges. Food Bioprocess Technol. 2024;17(11):3461 [Crossref]
26- Zalila-Kolsi I, Dhieb D, Osman H, Mekideche H. The Gut Microbiota and Colorectal Cancer: Understanding the Link and Exploring Therapeutic Interventions. Biology. 2025;14(3):251 [Crossref]
27- Yue S, Zhang Z, Bian F, Zhang Y, Chen G, Zhu Y, Li J, Picimbon J. 3L, Three-Lactobacilli on Recovering of Microbiome and Immune-Damage by Cyclophosphamide Chemotherapy—A Pilot Experiment in Rats. Microbiology Research. 2023;14(3):831 [Crossref]
28- Karam F, El Deghel Y, Iratni R, Dakroub A, Eid A. The Gut Microbiome and Colorectal Cancer: An Integrative Review of the Underlying Mechanisms. Cell Biochem Biophys. 2025; [Crossref]
29- Abedi A, Tafvizi F, Akbari N, Jafari P. Cell-Free Supernatant of L. buchneri Probiotic Bacteria Enhancing Apoptosis Activity in AGS Gastric Cancer Cells. Iran J Sci. 2023;47(4):1071 [Crossref]
30- Doublier S, Cirrincione S, Scardaci R, Botta C, Lamberti C, Giuseppe F, Angelucci S, Rantsiou K, Cocolin L, Pessione E. Putative probiotics decrease cell viability and enhance chemotherapy effectiveness in human cancer cells: role of butyrate and secreted proteins. Microbiological Research. 2022;260:127012 [Crossref]
31- Gholipour F, Amini M, Baradaran B, Mokhtarzadeh A, Eskandani M. Anticancer properties of curcumin-treated Lactobacillus plantarum against the HT-29 colorectal adenocarcinoma cells. Sci Rep. 2023;13(1) [Crossref]
32- Manoharan M, Ragothaman P, Balasubramanian T. Initiation of Apoptotic Pathway by the Cell-Free Supernatant Synthesized from Weissella cibaria Through In-Silico and In-Vitro Methods. Appl Biochem Biotechnol. 2024;196(7):4700 [Crossref]
33- Xu Y, Wu X, Li Y, Liu X, Fang L, Jiang Z. Probiotics and the Role of Dietary Substrates in Maintaining the Gut Health: Use of Live Microbes and Their Products for Anticancer Effects against Colorectal Cancer. J Microbiol Biotechnol. 2024;34(10):1933 [Crossref]
34- Jiang Y, Wu J, Tian L, Liu Y, Zhao F, He Z, Mao Y, Jia J, Guan T. The therapeutic effects of fermented milk with lactic acid bacteria from traditional Daqu on hypertensive mice. Journal of Dairy Science. 2024;107(2):742 [Crossref]
35- Nasreen S, Ali S, Andleeb S, Summer M, Hussain T, Imdad K, Ara C, Tahir H. Mechanisms of medicinal, pharmaceutical, and immunomodulatory action of probiotics bacteria and their secondary metabolites against disease management: an overview. Folia Microbiol. 2024;69(3):549 [Crossref]
36- Soleymanzadeh Moghadam S, Minaeian S, Majidpour A, Adabi M, Hosseini Doust R. The Lactobacillus acidophilus Supernatant: An Effective and Safe Alternative to Antibiotics. IJT. 2024;18(1):52 [Crossref]
37- Arun K, Sindhu R, Alex D, Binod P, Pughazhendi A, Joseph T, Pandey A, Kuddus M, Pillai S, Emmanual S, Awasthi M, Madhavan A. Bacterial bioactive metabolites as therapeutic agents: From production to action. Sustainable Chemistry and Pharmacy. 2022;27:100650 [Crossref]
38- Abedi A, Tafvizi F, Jafari P, Akbari N. The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis. Sci Rep. 2024;14(1) [Crossref]
39- Moskova-Doumanova V, Vaseva A, Veleva R, Mladenova K, Melniska D, Doumanov J, Videv P, Topouzova-Hristova T, Dobreva L, Atanasova N, Danova S. In Vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells. Microorganisms. 2024;12(7):1365 [Crossref]
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42- Zhu D, Pan W, Li H, Hua J, Zhang C, Zhao K. Innovative Applications of Bacteria and Their Derivatives in Targeted Tumor Therapy. ACS Nano. 2025;19(5):5077 [Crossref]

As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
Acceptance rate (2024): 20% 
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