Alireza Khoshroo
1* 
, Maryamosadat Mavaei
2, Masoume Rostami
3, Bahare Valinezhad-Saghezi
3, Ali Fattahi
2,4* 1 Nutrition Health Research center, Hamadan University of Medical Sciences, Hamadan, Iran
2 Pharmaceutical Sciences Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Student Research Committe, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Medical Biology Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Introduction: Bacterial infections have always been a major threat to public health and humans' life, and fast detection of bacteria in various samples is significant to provide early and effective treatments. Cell-culture protocols, as well-established methods, involve labor-intensive and complicated preparation steps. For overcoming this drawback, electrochemical methods may provide promising alternative tools for fast and reliable detection of bacterial infections.
Methods: Therefore, this review study was done to present an overview of different electrochemical strategy based on recognition elements for detection of bacteria in the studies published during 2015-2020. For this purpose, many references in the field were reviewed, and the review covered several issues, including (a) enzymes, (b) receptors, (c) antimicrobial peptides, (d) lectins, (e) redox-active metabolites, (f) aptamer, (g) bacteriophage, (h) antibody, and (i) molecularly imprinted polymers.
Results: Different analytical methods have developed are used to bacteria detection. However, most of these methods are highly time, and cost consuming, requiring trained personnel to perform the analysis. Among of these methods, electrochemical based methods are well accepted powerful tools for the detection of various analytes due to the inherent properties. Electrochemical sensors with different recognition elements can be used to design diagnostic system for bacterial infections. Recent studies have shown that electrochemical assay can provide promising reliable method for detection of bacteria.
Conclusion: In general, the field of bacterial detection by electrochemical sensors is continuously growing. It is believed that this field will focus on portable devices for detection of bacteria based on electrochemical methods. Development of these devices requires close collaboration of various disciplines, such as biology, electrochemistry, and biomaterial engineering.