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BioImpacts. 2022;12(6): 561-566.
doi: 10.34172/bi.2022.23825
PMID: 36644547
PMCID: PMC9809133
Scopus ID: 85143320810
  Abstract View: 940
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Short Communication

Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata

Maryam Moazeni 1,2 ORCID logo, Majid Saeedi 3 ORCID logo, Hamidreza Kelidari 3 ORCID logo, Behrad Roohi 4, Mohammad T Hedayati 1,2 ORCID logo, Tahereh Shokohi 1,2 ORCID logo, Mojtaba Nabili 5 ORCID logo, Kofi Asare-Addo 6 ORCID logo, Ali Nokhodchi 7,8* ORCID logo

1 Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3 Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4 Student Research Committee Center, Mazandaran University of Medical Sciences, Sari, Iran
5 Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran
6 Department of Pharmacy, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK
7 Pharmaceutics Research Laboratory, School of Life Sciences, University of Sussex, Arundel Building, Brighton BN1 9QJ, UK
8 Lupin Pharmaceutical Research Center, Coral Springs, Florida, USA
*Corresponding Author: Corresponding authors: Ali Nokhodchi,, Email: a.nokhodchi@sussex.ac.uk

Abstract

Introduction: This study was proposed to assess the potential role of efflux transporters in reversing fluconazole resistance in Candida glabrata isolates treated with fluconazole loaded nanostructured lipid carriers (FLZ-NLCs).
Methods: The ultrasound technique was used to synthesize the FLZ-NLCs. Four fluconazole-resistant, as well as one susceptible standard C. glabrata isolates, were applied and exposed to FLZ/ FLZ-NLCs for 20 h at 37°C. Real-time PCRs were done to estimate the likely changes in ATP-binding cassette transporter genes.
Results: Similar to the FLZ-exposed-susceptible standard strain which showed no alteration, the genes were not up-regulated significantly under the FLZ-NLCs treated condition. While they were over-expressed when the yeasts were treated with fluconazole.
Conclusion: It is highly suggested that due to the nature of the NLCs which shields the whole conformation of the drug, FLZ is not recognized by the efflux transporter subunits and consequently the translocation would not happen.
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Submitted: 09 May 2021
Revision: 14 Dec 2021
Accepted: 13 Feb 2022
ePublished: 30 Oct 2022
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