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Bioimpacts. 2016;6(1): 15-24. doi: 10.15171/bi.2016.03
PMID: 27340620        PMCID: PMC4916547

Original Research

Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture

Francesco Angelini 1 * , Vittoria Ionta 2, Fabrizio Rossi 2, Fabio Miraldi 3, Elisa Messina 4, Alessandro Giacomello 2

Cited by CrossRef: 27

1- Vigneault P, Parent S, Kanda P, Michie C, Davis D, Nattel S. Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection. Nat Commun. 2021;12(1) [Crossref]
2- Pham C, Midge S, Barua H, Zhang Y, Ngoc-Gia Nguyen T, Barrero R, Duan A, Yin W, Jiang G, Hou Y, Zhou S, Wang Y, Xie X, Tran P, Xiang D, Duan W. Bovine extracellular vesicles contaminate human extracellular vesicles produced in cell culture conditioned medium when ‘exosome-depleted serum’ is utilised. Archives of Biochemistry and Biophysics. 2021;708:108963 [Crossref]
3- Yu G, Jung H, Kang Y, Mok H. Comparative evaluation of cell- and serum-derived exosomes to deliver immune stimulators to lymph nodes. Biomaterials. 2018;162:71 [Crossref]
4- Jabbari N, Karimipour M, Khaksar M, Akbariazar E, Heidarzadeh M, Mojarad B, Aftab H, Rahbarghazi R, Rezaie J. Tumor-derived extracellular vesicles: insights into bystander effects of exosomes after irradiation. Lasers Med Sci. 2020;35(3):531 [Crossref]
5- Kornilov R, Puhka M, Mannerström B, Hiidenmaa H, Peltoniemi H, Siljander P, Seppänen‐Kaijansinkko R, Kaur S. Efficient ultrafiltration‐based protocol to deplete extracellular vesicles from fetal bovine serum. J of Extracellular Vesicle. 2018;7(1) [Crossref]
6- Lee J, Lee J, Mondal J, Hwang J, Kim H, Kumar V, Raj A, Hwang S, Lee Y. Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes. SSRN Journal. 2022; [Crossref]
7- Lee J, Lee J, Mondal J, Hwang J, Kim H, Kumar V, Raj A, Hwang S, Lee Y. Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes. IJMS. 2022;23(17):9619 [Crossref]
8- Lehrich B, Liang Y, Fiandaca M. Foetal bovine serum influence on in vitro extracellular vesicle analyses. J of Extracellular Vesicle. 2021;10(3) [Crossref]
9- Brown S, Dewitt S, Clayton A, Waddington R. Identifying the Efficacy of Extracellular Vesicles in Osteogenic Differentiation: An EV-Lution in Regenerative Medicine. Front Dent Med. 2022;3 [Crossref]
10- Urzì O, Olofsson Bagge R, Crescitelli R. The dark side of foetal bovine serum in extracellular vesicle studies. J of Extracellular Vesicle. 2022;11(10) [Crossref]
11- Shekari F, Alibhai F, Baharvand H, Börger V, Bruno S, Davies O, Giebel B, Gimona M, Salekdeh G, Martin‐Jaular L, Mathivanan S, Nelissen I, Nolte‐’t Hoen E, O'Driscoll L, Perut F, Pluchino S, Pocsfalvi G, Salomon C, Soekmadji C, Staubach S, Torrecilhas A, Shelke G, Tertel T, Zhu D, Théry C, Witwer K, Nieuwland R. Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters. J of Extracellular Bio. 2023;2(10) [Crossref]
12- Anji A, Anderson B, Akhtar F, Meekins D, Ito T, Mummidi S, Kumari M. Exosomes induce neurogenesis of pluripotent P19 cells. Stem Cell Rev and Rep. 2023;19(5):1152 [Crossref]
13- Chang Y, Wu K, Ding D. Chondrogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells Cultured with Exosome-Depleted Fetal Bovine Serum in an Osteoarthritis Mouse Model. Biomedicines. 2022;10(11):2773 [Crossref]
14- Inci F. Benchmarking a Microfluidic-Based Filtration for Isolating Biological Particles. Langmuir. 2022;38(5):1897 [Crossref]
15- Kahroba H, Davatgaran-Taghipour Y. Exosomal Nrf2: From anti-oxidant and anti-inflammation response to wound healing and tissue regeneration in aged-related diseases. Biochimie. 2020;171-172:103 [Crossref]
16- Busatto S, Yang Y, Iannotta D, Davidovich I, Talmon Y, Wolfram J. Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays. J of Extracellular Vesicle. 2022;11(4) [Crossref]
17- Majidinia M, Reiter R, Shakouri S, Mohebbi I, Rastegar M, Kaviani M, Darband S, Jahanban-Esfahlan R, Nabavi S, Yousefi B. The multiple functions of melatonin in regenerative medicine. Ageing Research Reviews. 2018;45:33 [Crossref]
18- Yan H, Li Y, Cheng S, Zeng Y. Advances in Analytical Technologies for Extracellular Vesicles. Anal Chem. 2021;93(11):4739 [Crossref]
19- Lee J, Kim H, Heo Y, Yoo Y, Han S, Kim C, Hur D, Kim H, Kang J, Lee J. Enhanced paper-based ELISA for simultaneous EVs/exosome isolation and detection using streptavidin agarose-based immobilization. Analyst. 2020;145(1):157 [Crossref]
20- Lehrich B, Liang Y, Fiandaca M. Response to “Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells”. J of Extracellular Vesicle. 2019;8(1) [Crossref]
21- Blaser M, Aikawa E. Roles and Regulation of Extracellular Vesicles in Cardiovascular Mineral Metabolism. Front Cardiovasc Med. 2018;5 [Crossref]
22- Bendinelli P, Maroni P, Dall’Olio V, Matteucci E, Desiderio M. Bone Metastasis Phenotype and Growth Undergo Regulation by Micro-Environment Stimuli: Efficacy of Early Therapy with HGF or TGFβ1-Type I Receptor Blockade. IJMS. 2019;20(10):2520 [Crossref]
23- Kazemzadeh M, Martinez-Calderon M, Otupiri R, Artuyants A, Lowe M, Ning X, Reategui E, Schultz Z, Xu W, Blenkiron C, Chamley L, Broderick N, Hisey C. Deep autoencoder as an interpretable tool for Raman spectroscopy investigation of chemical and extracellular vesicle mixtures. Biomed Opt Express. 2024;15(7):4220 [Crossref]
24- Shokouhy M, Sarvnaz H, Taslimi Y, Lajevardi M, Habibzadeh S, Mizbani A, Shekari F, Behbahani M, Torrecilhas A, Rafati S. Isolation, characterization, and functional study of extracellular vesicles derived from Leishmania tarentolae. Front Cell Infect Microbiol. 2022;12 [Crossref]

As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
Acceptance rate (2024): 20% 
Publication fee: Free of charge

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