Improvement in the stability and functionality of Nicotiana tabacum produced recombinant TRAIL through employment of endoplasmic reticulum expression and ascorbate buffer mediated extraction strategies

Hamid Reza Heidari ^1,2, Mojgan Bandehpour ^3,4, Hossein Vahidi ¹, Jaleh Barar ⁵ , Bahram Kazemi ^3,4 * , Hossein Naderi-Manesh ⁶ *

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1- Thiganta K, Rattanapisit K, Suwanchaikasem P, Roobsoong W, Bulaon C, Phoolcharoen W. Plant-produced monoclonal antibodies recognize the native circumsporozoite protein on Plasmodium falciparum sporozoites. Biotechnology Reports. 2026;49:e00944 [Crossref]

2- Bulaon C, Shanmugaraj B, Oo Y, Rattanapisit K, Chuanasa T, Chaotham C, Phoolcharoen W. Rapid transient expression of functional human vascular endothelial growth factor in Nicotiana benthamiana and characterization of its biological activity. Biotechnology Reports. 2020;27:e00514 [Crossref]

3- Hanittinan O, Oo Y, Chaotham C, Rattanapisit K, Shanmugaraj B, Phoolcharoen W. Expression optimization, purification and in vitro characterization of human epidermal growth factor produced in Nicotiana benthamiana. Biotechnology Reports. 2020;28:e00524 [Crossref]

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