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Bioimpacts. 2019;9(3): 131-144. doi: 10.15171/bi.2019.18
PMID: 31508329        PMCID: PMC6726745

Original Research

A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein

Mohammad Mostafa Pourseif 1,2, Mitra Yousefpour 1 * , Mohammad Aminianfar 2, Gholamali Moghaddam 3, Ahmad Nematollahi 4 ORCID

Cited by CrossRef: 46

1- Moghadaszadeh M, Khayyati M, Spotin A, Norouzi R, Pagheh A, Oliveira S, Pereira M, Ahmadpour E. Scolicidal and Apoptotic Activities of 5-hydroxy-1, 4-naphthoquinone as a Potent Agent against Echinococcus granulosus Protoscoleces. Pharmaceuticals. 2021;14(7):623 [Crossref]
2- Alissa M, Alghamdi A, Alghamdi S, Suleman M. Immunoinformatic based designing of highly immunogenic multi-epitope subunit vaccines to stimulate an adaptive immune response against Junin virus. Mol Divers. 2024; [Crossref]
3- N, Haq I, Rahiyab M, Ali S, Khan I, Iqbal A. Rational in-silico design of a multi-epitope vaccine against human Rhinovirus an immune simulation and molecular dynamics simulation approach. Vacunas. 2025;26(3):500427 [Crossref]
4- Nourmohammadi H, Javanmardi E, Shams M, Shamsinia S, Nosrati M, Yousefi A, Nemati T, Fatollahzadeh M, Ghasemi E, Kordi B, Majidiani H, Irannejad H. Multi-epitope vaccine against cystic echinococcosis using immunodominant epitopes from EgA31 and EgG1Y162 antigens. Informatics in Medicine Unlocked. 2020;21:100464 [Crossref]
5- Martinelli D. In silico vaccine design: A tutorial in immunoinformatics. Healthcare Analytics. 2022;2:100044 [Crossref]
6- Kharisma V, Ansori A. Construction of Epitope-Based Peptide Vaccine Against SARS-CoV-2: Immunoinformatics Study. J Pure Appl Microbiol. 2020;14(suppl 1):999 [Crossref]
7- Abdelmoneim A, Mustafa M, Abdelmageed M, Murshed N, Dawoud E, Ahmed E, Kamal Eldein R, Elfadol N, Sati A, Makhawi A. Immunoinformatics design of multiepitopes peptide-based universal cancer vaccine using matrix metalloproteinase-9 protein as a target. Immunological Medicine. 2021;44(1):35 [Crossref]
8- Mohammadzadeh R, Soleimanpour S, Pishdadian A, Farsiani H. Designing and development of epitope-based vaccines againstHelicobacter pylori. Critical Reviews in Microbiology. 2022;48(4):489 [Crossref]
9- Kuri P, Goswami P. Current Update on Rotavirus in-Silico Multiepitope Vaccine Design. ACS Omega. 2023;8(1):190 [Crossref]
10- Sheikh K, Khanam S, Salam N. In silico approach for a multi-epitope, multi-stage vaccine construct against human hookworm infection. Next Research. 2025;2(4):100805 [Crossref]
11- Kardani K, Bolhassani A, Namvar A. An overview ofin silicovaccine design against different pathogens and cancer. Expert Review of Vaccines. 2020;19(8):699 [Crossref]
12- Pourseif M, Masoudi-Sobhanzadeh Y, Azari E, Parvizpour S, Barar J, Ansari R, Omidi Y. Self-amplifying mRNA vaccines: Mode of action, design, development and optimization. Drug Discovery Today. 2022;27(11):103341 [Crossref]
13- N, Haq I, Rahiyab M, Ali S, Khan I, Iqbal A. Rational in-silico design of a multi-epitope vaccine against human Rhinovirus an immune simulation and molecular dynamics simulation approach. Vacunas (English Edition). 2025;26(3):500427 [Crossref]
14- Pourseif M, Moghaddam G, Nematollahi A, Khordadmehr M, Naghili B, Dehghani J, Omidi Y. Vaccination with rEGVac elicits immunoprotection against different stages of Echinococcus granulosus life cycle: A pilot study. Acta Tropica. 2021;218:105883 [Crossref]
15- Parvin R, Masum M, Heema H, Akter A, Hossain M, Siddiki A, Haque A. Designing of a multiepitope-based vaccine against echinococcosis utilizing the potent Ag5 antigen: Immunoinformatics and simulation approaches. PLoS ONE. 2025;20(2):e0310510 [Crossref]
16- Zhao Z, Ma X, Zhang R, Hu F, Zhang T, Liu Y, Han M, You F, Yang Y, Zheng W. A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals. Nanomedicine: Nanotechnology, Biology and Medicine. 2021;35:102338 [Crossref]
17- Chukwudozie O, Chukwuanukwu R, Iroanya O, Eze D, Duru V, Dele-Alimi T, Kehinde B, Bankole T, Obi P, Okinedo E. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research. 2020;2020:1 [Crossref]
18- Majidiani H, Pourseif M, Kordi B, Sadeghi M, Najafi A. TgVax452, an epitope-based candidate vaccine targeting Toxoplasma gondii tachyzoite-specific SAG1-related sequence (SRS) proteins: immunoinformatics, structural simulations and experimental evidence-based approaches. BMC Infect Dis. 2024;24(1) [Crossref]
19- Ji S, Gavande P, Choudhury B, Goyal A. Computational design and structure dynamics analysis of bifunctional chimera of endoxylanase from Clostridium thermocellum and xylosidase from Bacteroides ovatus. 3 Biotech. 2023;13(2) [Crossref]
20- TOPUZOĞULLARI M, ACAR T, PELİT ARAYICI P, UÇAR B, UĞUREL E, ABAMOR E, ARASOĞLU T, TURGUT-BALIK D, DERMAN S. An insight into the epitope-based peptide vaccine design strategy and studies against COVID-19. Turk J Biol. 2020;44(3):215 [Crossref]
21- Du X, Zhu M, Zhang T, Wang C, Tao J, Yang S, Zhu Y, Zhao W. The Recombinant Eg.P29-Mediated miR-126a-5p Promotes the Differentiation of Mouse Naive CD4+ T Cells via DLK1-Mediated Notch1 Signal Pathway. Front Immunol. 2022;13 [Crossref]
22- Kaushal N, Jain S, Baranwal M. Computational design of immunogenic peptide constructs comprising multiple human leukocyte antigen restricted dengue virus envelope epitopes. J of Molecular Recognition. 2022;35(9) [Crossref]
23- Simay S, Akbarzadeh-Khiavi M, Pourseif M, Barar J, Safary A, Omidi Y. Recombinant production and characterization of L-glutaminase (glsA) as a promiscuity therapeutic enzyme. Appl Microbiol Biotechnol. 2022;106(17):5511 [Crossref]
24- Zhou B, Ding H, Yang J, Chai J, Guo H, Wang H. Effects of diclazuril on the expression of enolase in second-generation merozoites of Eimeria tenella. Poultry Science. 2020;99(12):6402 [Crossref]
25- Sadr S, Borji H. Echinococcus granulosus as a Promising Therapeutic Agent against Triplenegative Breast Cancer. CCTR. 2023;19(4):292 [Crossref]
26- Halder S, Sharma A, Al Arian T, Saha S, Shil A, Rafi M, Sarker S, Alam M, Himel M, Hasan M, Shawan M. In silico Designing of a Multi-epitope-based Subunit Vaccine against SARS-CoV-2 (Delta Variant) by Exploiting Its Structural Proteins: A Reverse Vaccinomics and Immunoinformatics Approach. COVID. 2024;5(4) [Crossref]
27- Rajput V, Sharma R, Kumari A, Vyas N, Prajapati V, Grover A. Engineering a multi epitope vaccine against SARS-CoV-2 by exploiting its non structural and structural proteins. Journal of Biomolecular Structure and Dynamics. 2022;40(19):9096 [Crossref]
28- Salaikumaran M, Kasamuthu P, Aathmanathan V, Burra V. An in silico approach to study the role of epitope order in the multi-epitope-based peptide (MEBP) vaccine design. Sci Rep. 2022;12(1) [Crossref]
29- Kafle A, Ojha S. Advancing vaccine development against Opisthorchis viverrini: A synergistic integration of omics technologies and advanced computational tools. Front Pharmacol. 2024;15 [Crossref]
30- Ahmed M, Rao T, Mutahir Z, Ahmed S, Ullah N, Ojha S. Immunoinformatic-driven design and evaluation of multi-epitope mRNA vaccine targeting HIV-1 gp120. Front Immunol. 2025;16 [Crossref]
31- Fathollahi M, Fathollahi A, Motamedi H, Moradi J, Alvandi A, Abiri R. In silico vaccine design and epitope mapping of New Delhi metallo-beta-lactamase (NDM): an immunoinformatics approach. BMC Bioinformatics. 2021;22(1) [Crossref]
32- Rahiyab M, Ul Haq I, Ali S, Hussain Z, Ali S, Khan I, Iqbal A. Design of a new multi-epitope subunit vaccine to combat the EIA virus, targeting Pol, Gag, and Env proteins: In silico technique. Vacunas. 2025;26(3):500463 [Crossref]
33- Saha R, Ghosh P, Burra V. Designing a next generation multi-epitope based peptide vaccine candidate against SARS-CoV-2 using computational approaches. 3 Biotech. 2021;11(2) [Crossref]
34- Goodswen S, Kennedy P, Ellis J. A guide to current methodology and usage of reverse vaccinology towards in silico vaccine discovery. 2023;47(2) [Crossref]
35- Mugunthan S, Harish M. Multi-epitope-Based Vaccine Designed by Targeting Cytoadherence Proteins of Mycoplasma gallisepticum. ACS Omega. 2021;6(21):13742 [Crossref]
36- Yaghoobizadeh F, Roayaei Ardakani M, Ranjbar M, Khosravi M, Galehdari H. Development of a potent recombinant scFv antibody against the SARS-CoV-2 by in-depth bioinformatics study: Paving the way for vaccine/diagnostics development. Computers in Biology and Medicine. 2024;170:108091 [Crossref]
37- de Araújo L, de Melo Santos N, Corsetti P, de Almeida L. Immunoinformatic Approach for Rational Identification of Immunogenic Peptides Against Host Entry and/or Exit Mpox Proteins and Potential Multiepitope Vaccine Construction. 2024;229(Supplement_2):S285 [Crossref]
38- Rahiyab M, Ul Haq I, Ali S, Hussain Z, Ali S, Khan I, Iqbal A. Design of a new multi-epitope subunit vaccine to combat the EIA virus, targeting Pol, Gag, and Env proteins: In silico technique. Vacunas (English Edition). 2025;26(3):500463 [Crossref]
39- Suleman M, Khan S, Rashid F, Khan A, Hussain Z, Zaman N, Rehman S, Zhai J, Xue M, Zheng C. Designing a multi-epitopes subunit vaccine against human herpes virus 6A based on molecular dynamics and immune stimulation. International Journal of Biological Macromolecules. 2023;244:125068 [Crossref]
40- Nath P, Goyal A. Structure and dynamics analysis of multi-domain putative β-1,4-glucosidase of family 3 glycoside hydrolase (PsGH3) from Pseudopedobacter saltans. J Mol Model. 2021;27(4) [Crossref]

As a peer-reviewed international open-access journal, BioImpacts publishes articles on basic and translational aspects of pharmaceutical and biomedical sciences. 
Acceptance rate (2024): 20% 
Publication fee: Free of charge

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