Bioimpacts. 2019;9(3):169-178.
doi: 10.15171/bi.2019.21
  Abstract View: 38
  PDF Download: 1

Original Research

The Effects of Natural Nano-Sized Clinoptilolite and Nigella Sativa Supplementation on Serum Bone Markers in Diabetic Rats.


Introduction: Many studies confirm that diabetes mellitus is associated with higher risks of bone fracture. The beneficial effects of nigella sativa (NS) and clinoptilolite in preventing/reducing some diabetes-related disorders have been shown. This study was conducted to examine the effects of separate and concurrent supplementation of natural nano-sized clinoptilolite (NCLN) and NS on serum bone markers in diabetic rats. Method: A total of 44 (case=36 and control=6) adult male Wistar rats were divided into two groups: diabetic and non-diabetic. An oral glucose tolerance test and a homeostatic model assessment of insulin resistance (HOMA-IR) test were conducted to confirm diabetes. Then, the diabetic group was divided into 4 subgroups: [1] control (n=9), [2] NS 1%/food (n=9), [3] NCLN 2%/food (n=9), [4] NS 1%/food + NCLN 2%/food (n=9). After 7 weeks, serum bone markers levels were determined using an ELISA kit. Results: Analysis showed that ALP was increased significantly in the group taking NCLN (P<0.05), compared to the normal-control (P=0.026) groups. Analysis showed that alkaline phosphatase serum level, in the group which took the NCLN supplement was significantly higher than in other groups (P<0.05). On the other hand, rats supplemented with NCLN+NS had significant higher serum levels of calcium (P=0.027) compared to all study groups. However, rats which consumed NS powder had higher PTH compared to other supplementation groups (P<0.0001). There were no significant differences in vitamin D and osteoprotegerin. Conclusion: The results of the current study suggest that bone mineralization may be affected by concurrent use of NS and NCLN through influencing calcium circulation. Moreover, dietary NS administration is strongly related to an augmented level of PTH.
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Submitted: 06 Aug 2018
Revised: 25 Jan 2019
Accepted: 26 Jan 2019
First published online: 15 Apr 2019
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