1. Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells

Sevda Rahimzadeh , Reza Rahbarghazi, Somayeh Aslani, Hadi Rajabi, Zeinab Latifi, Majid Farshdousti Hagh, Alireza Nourazarian, Hojjatollah Nozad Charoudeh, Mohammad Nouri* , Alireza Abhari*

Bioimpacts. 2020;10(4): 243-250. doi: 10.34172/bi.2020.31

PMCID: PMC7502906 PMID: 32983940 Scopus ID: 85092056978

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Adipose-derived mesenchymal stem cells were incubated with osteogenic differentiation medium. RNA and DNA were extracted on days 0, 7, 14 and 21 days of differentiation period. The expression and promoter methylation of DLX3, ATF4, and FRA1 genes were analyzed by real-time polymerase chain reaction and methylation-specific quantitative PCR, respectively

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